TRPC4 (TRP4;alpha, beta, gamma isoforms) (4th cytoplasmic loop) antibody

Antigen: TRPC4 (TRP4;alpha, beta, gamma isoforms) (4th cytoplasmic loop)

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN351144

Additional Information

Swiss-Prot: Q9QUQ5

Immunogen: A synthetic peptide from the 4th cytoplasmic loop of human TRPC4 (TRP4, alpha, beta, gamma isoforms) conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: FUNCTION: Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Has also been shown to be calcium-selective. May also be activated by intracellular calcium store depletion. TISSUE SPECIFICITY: Strongly expressed in placenta. Expressed at lower levels in heart, pancreas, kidney and brain. Isoform alpha was found to be the predominant isoform. Isoform beta was not found in pancreas and brain. Also known as: Short transient receptor potential channel 4, TrpC4, Trp4, Capacitative calcium entry channel 1, CCE1, Trrp4, Receptor-activated cation channel TRP4, Trp-related protein 4, hTrp-4, hTrp4.

Specificity: Appears to be specific for TRPC4.

Application Details

Application Notes: IHC, WB. A dilution of 1: 300 to 1: 2000 is recommended. The optimal dilution should be determined by the end user.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Zhu, Jiang, Peyton et al.: "trp, a novel mammalian gene family essential for agonist-activated capacitative Ca2+ entry." in: Cell, Vol. 85, Issue 5, pp. 661-71, 1996 (PubMed).

McKay, Szymeczek-Seay, Lievremont et al.: "Cloning and expression of the human transient receptor potential 4 (TRP4) gene: localization and functional expression of human TRP4 and TRP3." in: The Biochemical journal, Vol. 351 Pt 3, pp. 735-46, 2001 (PubMed).

Mery, Magnino, Schmidt et al.: "Alternative splice variants of hTrp4 differentially interact with the C-terminal portion of the inositol 1,4,5-trisphosphate receptors." in: FEBS letters, Vol. 487, Issue 3, pp. 377-83, 2001 (PubMed).

Schaefer, Plant, Stresow et al.: "Functional differences between TRPC4 splice variants." in: The Journal of biological chemistry, Vol. 277, Issue 5, pp. 3752-9, 2002 (PubMed).

Sjoeblom, Jones, Wood et al.: "The consensus coding sequences of human breast and colorectal cancers." in: Science (New York, N.Y.), Vol. 314, Issue 5797, pp. 268-74, 2006 (PubMed).

Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).