TRPC6, 3, 7 (4th Cytoplasmic Loop) antibody

Antigen: TRPC6, 3, 7

Binding Site: 4th Cytoplasmic Loop

Clonality: Polyclonal

Host: Rabbit

Reactivity: Mouse (Murine)

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN351162

Additional Information

UniProt: Q61143

Immunogen: A synthetic peptide from the 4th cytoplasmic loop of mouse TRPC6 conjugated to an immunogenic carrier protein was used as the immunogen. The antigen shares 94% identity with TRPC3 and TRPC7 sequences.

Format: Lyophilized

Description: FUNCTION: Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C. Seems not to be activated by intracellular calcium store depletion. Tissue specificity: Lung and brain. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. Also known as: Short transient receptor potential channel 6, TrpC6, Calcium entry channel, Trp6, transient receptor potential cation channel, subfamily C, member 6, Trrp6, mtrp6.

Specificity: Appears to recognise the TRPC6, TRPC3 and TRPC7.

Application Details

Application Notes: IHC, WB. A dilution of 1: 300 to 1: 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Zhu, Jiang, Peyton et al.: "trp, a novel mammalian gene family essential for agonist-activated capacitative Ca2+ entry." in: Cell, Vol. 85, Issue 5, pp. 661-71, 1996 (PubMed).

Boulay, Zhu, Peyton et al.: "Cloning and expression of a novel mammalian homolog of Drosophila transient receptor potential (Trp) involved in calcium entry secondary to activation of receptors coupled by the Gq class of G protein." in: The Journal of biological chemistry, Vol. 272, Issue 47, pp. 29672-80, 1997 (PubMed).

Buess, Engler, Hirsch et al.: "Search for oncogenic regulators in an autocrine tumor model using differential display PCR: identification of novel candidate genes including the calcium channel mtrp6." in: Oncogene, Vol. 18, Issue 7, pp. 1487-94, 1999 (PubMed).

Ramsey, Delling, Clapham: "An introduction to TRP channels." in: Annual review of physiology, Vol. 68, pp. 619-47, 2006 (PubMed).

Weissmann, Dietrich, Fuchs et al.: "Classical transient receptor potential channel 6 (TRPC6) is essential for hypoxic pulmonary vasoconstriction and alveolar gas exchange." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 50, pp. 19093-8, 2006 (PubMed).

Dietrich, Gudermann: "TRPC6." in: Handbook of experimental pharmacology, Issue 179, pp. 125-41, 2007 (PubMed).