Transient Receptor Potential Cation Channel, Subfamily C, Member 6 (TRPC6) (4th cytoplasmic loop) antibody

Antigen: Transient Receptor Potential Cation Channel, Subfamily C, Member 6 (TRPC6)

Synonyms: TRP6, FSGS2, FLJ11098, FLJ14863, Trrp6, mtrp6, AV025995, LLHWJM002, LLHWJM003, LLHWJM004, TRPC6, trp6, trpc6, bZ1P14.9, si:rp71-1p14.9, Trpc6

Epitope: 4th cytoplasmic loop

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN351167

Additional Information

Alternative name: TRPC6, 3, 7 (4th cytoplasmic loop)

Swiss-Prot: Q9Y210

Immunogen: A synthetic peptide from the 4th cytoplasmic loop of human TRPC6 conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: FUNCTION: Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C. Seems not to be activated by intracellular calcium store depletion. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. Also known as: Short transient receptor potential channel 6, TRP6, transient receptor potential cation channel, subfamily C, member 6, TRP6.

Specificity: Appears to recognise TRPC6, TRPC3 and TRPC7.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: DEsposito, Strazzullo, Cuccurese et al.: "Identification and assignment of the human transient receptor potential channel 6 gene TRPC6 to chromosome 11q21-->q22." in: Cytogenetics and cell genetics, Vol. 83, Issue 1-2, pp. 46-7, 1999 (PubMed).

Hofmann, Obukhov, Schaefer et al.: "Direct activation of human TRPC6 and TRPC3 channels by diacylglycerol." in: Nature, Vol. 397, Issue 6716, pp. 259-63, 1999 (PubMed).

Philipp, Trost, Warnat et al.: "TRP4 (CCE1) protein is part of native calcium release-activated Ca2+-like channels in adrenal cells." in: The Journal of biological chemistry, Vol. 275, Issue 31, pp. 23965-72, 2000 (PubMed).

Winn, Conlon, Lynn et al.: "A mutation in the TRPC6 cation channel causes familial focal segmental glomerulosclerosis." in: Science (New York, N.Y.), Vol. 308, Issue 5729, pp. 1801-4, 2005 (PubMed).

Reiser, Polu, Moeller et al.: "TRPC6 is a glomerular slit diaphragm-associated channel required for normal renal function." in: Nature genetics, Vol. 37, Issue 7, pp. 739-44, 2005 (PubMed).

Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).