TRPC6, 3, 7 (4th Cytoplasmic Domain) antibody

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4th Cytoplasmic Domain
Immunohistochemistry (IHC), Western Blotting (WB)
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Immunogen A synthetic peptide from 4th cytoplasmic domain of human TRPC6, 3, 7 conjugated to an immunogenic carrier protein was used as the antigen.
Specificity Appears to recognise TRPC6, TRPC3 and TRPC7.
Purification Whole serum
Background Function: Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C. Seems not to be activated by intracellular calcium store depletion.
Subcellular location: Membrane, Multi-pass membrane protein (Probable). Also known as: Short transient receptor potential channel 6, TRP6, transient receptor potential cation channel, subfamily C, member 6, TRP6, FSGS2, FLJ11098, FLJ14863.
Application Notes A dilution of 1 : 300 to 1 : 2000 is recommended.
The optimal dilution should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Background publications Olsen, Blagoev, Gnad, Macek, Kumar, Mortensen, Mann: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).

Winn, Conlon, Lynn, Farrington, Creazzo, Hawkins, Daskalakis, Kwan, Ebersviller, Burchette, Pericak-Vance, Howell, Vance, Rosenberg: "A mutation in the TRPC6 cation channel causes familial focal segmental glomerulosclerosis." in: Science (New York, N.Y.), Vol. 308, Issue 5729, pp. 1801-4, 2005 (PubMed).

Reiser, Polu, Möller, Kenlan, Altintas, Wei, Faul, Herbert, Villegas, Avila-Casado, McGee, Sugimoto, Brown, Kalluri, Mundel, Smith, Clapham, Pollak: "TRPC6 is a glomerular slit diaphragm-associated channel required for normal renal function." in: Nature genetics, Vol. 37, Issue 7, pp. 739-44, 2005 (PubMed).

Philipp, Trost, Warnat, Rautmann, Himmerkus, Schroth, Kretz, Nastainczyk, Cavalie, Hoth, Flockerzi: "TRP4 (CCE1) protein is part of native calcium release-activated Ca2+-like channels in adrenal cells." in: The Journal of biological chemistry, Vol. 275, Issue 31, pp. 23965-72, 2000 (PubMed).

DEsposito, Strazzullo, Cuccurese, Spalluto, Rocchi, DUrso, Ciccodicola: "Identification and assignment of the human transient receptor potential channel 6 gene TRPC6 to chromosome 11q21-->q22." in: Cytogenetics and cell genetics, Vol. 83, Issue 1-2, pp. 46-7, 1999 (PubMed).

Hofmann, Obukhov, Schaefer, Harteneck, Gudermann, Schultz: "Direct activation of human TRPC6 and TRPC3 channels by diacylglycerol." in: Nature, Vol. 397, Issue 6716, pp. 259-63, 1999 (PubMed).

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