Transient Receptor Potential Cation Channel, Subfamily C, Member 6 (TRPC6) (4th cytoplasmic loop) antibody

Antigen: Transient Receptor Potential Cation Channel, Subfamily C, Member 6 (TRPC6)

Synonyms: TRP6, FSGS2, FLJ11098, FLJ14863, Trrp6, mtrp6, AV025995, LLHWJM002, LLHWJM003, LLHWJM004, TRPC6, trp6, trpc6, bZ1P14.9, si:rp71-1p14.9, Trpc6

Epitope: 4th cytoplasmic loop

Clonality: Polyclonal

Host: Sheep

Reactivity: Rat (Rattus)

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN351169

Additional Information

Alternative name: TRPC6, 3, 7 (4th cytoplasmic loop)

Swiss-Prot: Q99N78

Immunogen: A synthetic peptide from 4th cytoplasmic domain of rat TRPC6, conjugated to an immunogenic carrier protein was used as the immunogen. The peptide is homologous with the corresponding sequence derived from TRP6A, 6B and 6C proteins.

Format: Lyophilized

Description: This is a G-protein-coupled receptor regulated Ca(2+) channel. Also known as: Transient receptor potential Ca2+ channel 6A, trp6A, transient receptor potential cation channel, subfamily C, member 6.

Specificity: Appears to recognise TRPC3, TRPC6 and TRPC7.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Zhang, Saffen: "Muscarinic acetylcholine receptor regulation of TRP6 Ca2+ channel isoforms. Molecular structures and functional characterization." in: The Journal of biological chemistry, Vol. 276, Issue 16, pp. 13331-9, 2001 (PubMed).

Jung, Strotmann, Schultz et al.: "TRPC6 is a candidate channel involved in receptor-stimulated cation currents in A7r5 smooth muscle cells." in: American journal of physiology. Cell physiology, Vol. 282, Issue 2, pp. C347-59, 2002 (PubMed).

Welsh, Morielli, Nelson et al.: "Transient receptor potential channels regulate myogenic tone of resistance arteries." in: Circulation research, Vol. 90, Issue 3, pp. 248-50, 2002 (PubMed).

Yu, Fantozzi, Remillard et al.: "Enhanced expression of transient receptor potential channels in idiopathic pulmonary arterial hypertension." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 38, pp. 13861-6, 2004 (PubMed).

Soboloff, Spassova, Xu et al.: "Role of endogenous TRPC6 channels in Ca2+ signal generation in A7r5 smooth muscle cells." in: The Journal of biological chemistry, Vol. 280, Issue 48, pp. 39786-94, 2005 (PubMed).

Lemos, Poburko, Liao et al.: "Na+ entry via TRPC6 causes Ca2+ entry via NCX reversal in ATP stimulated smooth muscle cells." in: Biochemical and biophysical research communications, Vol. 352, Issue 1, pp. 130-4, 2006 (PubMed).

Nishida, Onohara, Sato et al.: "Galpha12/13-mediated up-regulation of TRPC6 negatively regulates endothelin-1-induced cardiac myofibroblast formation and collagen synthesis through nuclear factor of activated T cells activation." in: The Journal of biological chemistry, Vol. 282, Issue 32, pp. 23117-28, 2007 (PubMed).