Transient Receptor Potential Cation Channel, Subfamily C, Member 7 (TRPC7) (1st cytoplasmic loop) antibody

Antigen: Transient Receptor Potential Cation Channel, Subfamily C, Member 7 (TRPC7)

Synonyms: TRP7, TRP-7, Trrp8, TRPC7, TRPM2

Epitope: 1st cytoplasmic loop

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN351170

Additional Information

Alternative name: TRPC7 (TRP7) (1t cytoplasmic loopl)

Swiss-Prot: Q9HCX4

Immunogen: A synthetic peptide from 1t cytoplasmic loop of human TRPC7 conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in mouse.

Format: Lyophilized

Description: FUNCTION: Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Activated by diacylglycerol (DAG). May also be activated by intracellular calcium store depletion. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. Also known as: Short transient receptor potential channel 7, TrpC7, mTRP7, Trp7, Trrp8, Trpc7, TrpC7, TRP7 protein, TRP7.

Specificity: Appears to be specific for TRPC7.

Application Details

Application Notes: IHC, WB (confirmed by recombinant protein). A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Liuevremont, Bird, Putney: "Canonical transient receptor potential TRPC7 can function as both a receptor- and store-operated channel in HEK-293 cells." in: American journal of physiology. Cell physiology, Vol. 287, Issue 6, pp. C1709-16, 2004 (PubMed).

Zagranichnaya, Wu, Villereal: "Endogenous TRPC1, TRPC3, and TRPC7 proteins combine to form native store-operated channels in HEK-293 cells." in: The Journal of biological chemistry, Vol. 280, Issue 33, pp. 29559-69, 2005 (PubMed).

Lievremont, Numaga, Vazquez et al.: "The role of canonical transient receptor potential 7 in B-cell receptor-activated channels." in: The Journal of biological chemistry, Vol. 280, Issue 42, pp. 35346-51, 2005 (PubMed).

Clapham, Julius, Montell et al.: "International Union of Pharmacology. XLIX. Nomenclature and structure-function relationships of transient receptor potential channels." in: Pharmacological reviews, Vol. 57, Issue 4, pp. 427-50, 2005 (PubMed).

Numaga, Wakamori, Mori: "TRPC7." in: Handbook of experimental pharmacology, Issue 179, pp. 143-51, 2007 (PubMed).