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TRPG (4th Cytoplasmic Loop) antibody

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4th Cytoplasmic Loop
Fruit Fly (Drosophila melanogaster)
Immunohistochemistry (IHC), Western Blotting (WB)
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Immunogen A synthetic peptide from 4th cytoplasmic loop of drosophila TRPG (Transient receptor potential-gamma protein, TRP gamma) conjugated to an immunogenic carrier protein was used as the antigen.
Isotype IgG
Specificity Specific for TRPG.
Background Function: A light-sensitive calcium channel that is required for inositide-mediated Ca(2+) entry in the retina during phospholipase C (PLC)-mediated phototransduction. Forms a regulated cation channel when heteromultimerized with trpl.
Subunit: Interacts preferentially with trpl and interacts to a lower extent with trp.
Subcellular location: Membrane, Multi-pass membrane protein.
Tissue specificity: Expressed predominantly in the rhabdomeres of photoreceptor cells. Also known as: TRPG, Transient receptor potential-gamma protein, TRPgamma.
Application Notes A concentration of 10-50 µg/ml is recommended.
The optimal concentration should be determined by the end user.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Background publications Xu, Chien, Butler, Salkoff, Montell: "TRPgamma, a drosophila TRP-related subunit, forms a regulated cation channel with TRPL." in: Neuron, Vol. 26, Issue 3, pp. 647-57, 2000

Adams, Celniker, Holt, Evans, Gocayne, Amanatides, Scherer, Li, Hoskins, Galle, George, Lewis, Richards, Ashburner, Henderson, Sutton, Wortman, Yandell, Zhang, Chen, Brandon, Rogers, Blazej, Champe, Pfeiffer, Wan, Doyle, Baxter, Helt, Nelson, Gabor, Abril: "The genome sequence of Drosophila melanogaster." in: Science (New York, N.Y.), Vol. 287, Issue 5461, pp. 2185-95, 2000