Transient Receptor Potential Cation Channel, Subfamily C, Member 7 (TRPC7) (4th cytoplasmic domain) antibody

Antigen: Transient Receptor Potential Cation Channel, Subfamily C, Member 7 (TRPC7)

Synonyms: TRP7, TRP-7, Trrp8, TRPC7, TRPM2

Epitope: 4th cytoplasmic domain

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN351192

Additional Information

Alternative name: TRPM2 (TRPC7, LTRPC2) (4th (cytoplasmic domain))

Swiss-Prot: O94759

Immunogen: A synthetic peptide from the 4th cytoplasmic domain of human TRPM2 (TRPC7, LTRPC2) conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: The protein encoded by this gene is a calcium-permeable cation channel that is regulated by free intracellular ADP-ribose. The encoded protein is activated by oxidative stress and confers susceptibility to cell death. Several alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. FUNCTION: Nonselective, voltage-independent cation channel mediating sodium and calcium ion influx in response to oxidative stress. Extracellular calcium passes through the channel and acts from the intracellular side as a positive regulator in channel activation. Activated by ADP-ribose, nicotinamide adenine dinucleotide (NAD(+)), reactive nitrogen species and arachidonic acid. Inactivated by intracellular ATP. Confers susceptibility to cell death following oxidative stress. CATALYTIC ACTIVITY: ADP-ribose + H2O = AMP + D-ribose 5-phosphate. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. Also known as: Transient receptor potential cation channel subfamily M member 2, long transient receptor potential channel 2, LTrpC2, LTrpC-2, transient receptor potential channel 7, TrpC7, estrogen-responsive element-associated gene 1 protein, EREG1, KNP3, LTRPC2, TRPC7, KNP3, NUDT9H, NUDT9L1.

Specificity: Appears to be specific for TRPM2.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Nagamine, Kudoh, Minoshima et al.: "Molecular cloning of a novel putative Ca2+ channel protein (TRPC7) highly expressed in brain." in: Genomics, Vol. 54, Issue 1, pp. 124-31, 1999 (PubMed).

Hattori, Fujiyama, Taylor et al.: "The DNA sequence of human chromosome 21." in: Nature, Vol. 405, Issue 6784, pp. 311-9, 2000 (PubMed).

Perraud, Fleig, Dunn et al.: "ADP-ribose gating of the calcium-permeable LTRPC2 channel revealed by Nudix motif homology." in: Nature, Vol. 411, Issue 6837, pp. 595-9, 2001 (PubMed).

Sano, Inamura, Miyake et al.: "Immunocyte Ca2+ influx system mediated by LTRPC2." in: Science (New York, N.Y.), Vol. 293, Issue 5533, pp. 1327-30, 2001 (PubMed).

Hara, Wakamori, Ishii et al.: "LTRPC2 Ca2+-permeable channel activated by changes in redox status confers susceptibility to cell death." in: Molecular cell, Vol. 9, Issue 1, pp. 163-73, 2002 (PubMed).

Wehage, Eisfeld, Heiner et al.: "Activation of the cation channel long transient receptor potential channel 2 (LTRPC2) by hydrogen peroxide. A splice variant reveals a mode of activation independent of ADP-ribose." in: The Journal of biological chemistry, Vol. 277, Issue 26, pp. 23150-6, 2002 (PubMed).

Zhang, Chu, Tong et al.: "A novel TRPM2 isoform inhibits calcium influx and susceptibility to cell death." in: The Journal of biological chemistry, Vol. 278, Issue 18, pp. 16222-9, 2003 (PubMed).