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|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Transient Receptor Potential Cation Channel, Subfamily M, Member 2 (TRPM2) (4th Cytoplasmic Domain) antibody
|Synonyms||KNP3, EREG1, TRPC7, LTRPC2, NUDT9H, NUDT9L1, MGC133383, Trpm2-predicted, TRPM2, Trp7, Trrp7, C79133, 9830168K16Rik|
4th Cytoplasmic Domain
Alternatives Immunohistochemistry (IHC), Western Blotting (WB)
|7 references available|
|Price||454.67 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 7 to 8 Business Days|
|Alternative name||TRPM2 (TRPC7, LTRPC2)|
|Immunogen||A synthetic peptide from the 4th cytoplasmic domain of human TRPM2 (TRPC7, LTRPC2) conjugated to an immunogenic carrier protein was used as the immunogen.|
|Description||The protein encoded by this gene is a calcium-permeable cation channel that is regulated by free intracellular ADP-ribose. The encoded protein is activated by oxidative stress and confers susceptibility to cell death. Several alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. FUNCTION: Nonselective, voltage-independent cation channel mediating sodium and calcium ion influx in response to oxidative stress. Extracellular calcium passes through the channel and acts from the intracellular side as a positive regulator in channel activation. Activated by ADP-ribose, nicotinamide adenine dinucleotide (NAD(+)), reactive nitrogen species and arachidonic acid. Inactivated by intracellular ATP. Confers susceptibility to cell death following oxidative stress. CATALYTIC ACTIVITY: ADP-ribose + H2O = AMP + D-ribose 5-phosphate. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. Also known as: Transient receptor potential cation channel subfamily M member 2, long transient receptor potential channel 2, LTrpC2, LTrpC-2, transient receptor potential channel 7, TrpC7, estrogen-responsive element-associated gene 1 protein, EREG1, KNP3, LTRPC2, TRPC7, KNP3, NUDT9H, NUDT9L1.|
|Specificity||Appears to be specific for TRPM2.|
|Application Notes||IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.|
|Storage||Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.|
|Restrictions||For Research Use only|
Nagamine, Kudoh, Minoshima et al.: "Molecular cloning of a novel putative Ca2+ channel protein (TRPC7) highly expressed in brain." in: Genomics, Vol. 54, Issue 1, pp. 124-31, 1999 (PubMed).
Hattori, Fujiyama, Taylor et al.: "The DNA sequence of human chromosome 21." in: Nature, Vol. 405, Issue 6784, pp. 311-9, 2000 (PubMed).
Perraud, Fleig, Dunn et al.: "ADP-ribose gating of the calcium-permeable LTRPC2 channel revealed by Nudix motif homology." in: Nature, Vol. 411, Issue 6837, pp. 595-9, 2001 (PubMed).
Sano, Inamura, Miyake et al.: "Immunocyte Ca2+ influx system mediated by LTRPC2." in: Science (New York, N.Y.), Vol. 293, Issue 5533, pp. 1327-30, 2001 (PubMed).
Hara, Wakamori, Ishii et al.: "LTRPC2 Ca2+-permeable channel activated by changes in redox status confers susceptibility to cell death." in: Molecular cell, Vol. 9, Issue 1, pp. 163-73, 2002 (PubMed).
Wehage, Eisfeld, Heiner et al.: "Activation of the cation channel long transient receptor potential channel 2 (LTRPC2) by hydrogen peroxide. A splice variant reveals a mode of activation independent of ADP-ribose." in: The Journal of biological chemistry, Vol. 277, Issue 26, pp. 23150-6, 2002 (PubMed).
Zhang, Chu, Tong et al.: "A novel TRPM2 isoform inhibits calcium influx and susceptibility to cell death." in: The Journal of biological chemistry, Vol. 278, Issue 18, pp. 16222-9, 2003 (PubMed).
|Hosts||Rabbit (34), Goat (2), Sheep (2), Mouse (1)|
|Reactivities||Human (31), Rat (Rattus) (25), Mouse (Murine) (23), Horse (Equine) (18), Cow (Bovine) (1)|
|Applications||Western Blotting (WB) (21), Immunofluorescence (IF) (17), Flow Cytometry (FACS) (15), Immunohistochemistry (IHC) (10), ELISA (7), Immunoprecipitation (IP) (5), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunocytochemistry (ICC) (2), Immunoelectron Microscopy (IEM) (1)|
|Conjugates||Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)|
|Epitopes||C-Term (6), 3rd Extracellular Loop (1), 4th Cytoplasmic Domain (1), AA 1213-1265 (1), AA 845-862 (1), Internal Region (1), N-Term (1)|