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|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Transient Receptor Potential Cation Channel, Subfamily M, Member 4 (TRPM4) (C-Term) antibody
|Synonyms||TRPM4B, FLJ20041, LTRPC4, LTrpC-4, AW047689, 1110030C19Rik, Mls2s, trpm4, TRPM4|
Alternatives Immunohistochemistry (IHC), Western Blotting (WB)
|10 references available|
|Price||454.67 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 7 to 8 Business Days|
|Alternative name||TRPM4 (Melastatin-4)|
|Immunogen||A synthetic peptide as a part of human transient receptor potential cation channel subfamily M member 4 (TRPM4, melastatin-4) conjugated to immunogenic carrier protein was used as the immunogen.|
|Description||FUNCTION: Calcium-activated non selective (CAN) cation channel that mediates membrane depolarization. While it is activated by increase in intracellular Ca(2+), it is impermeable to it. Mediates transport of monovalent cations (Na(+) > K(+) > Cs(+) > Li(+)), leading to depolarize the membrane. It thereby plays a central role in cadiomyocytes, neurons from entorhinal cortex, dorsal root andvomeronasal neurons, endocrine pancreas cells, kidney epithelial cells, cochlea hair cells etc. Participates in T-cell activation by modulating Ca(2+) oscillations after T lymphocyte activation, which is required for NFAT-dependent IL2 production. Involved in myogenic constriction of cerebral arteries. Controls insulin secretion in pancreatic beta-cells. May also be involved in pacemaking or could cause irregular electrical activity under conditions of Ca(2+) overload. ENZYME REGULATION: Gating is voltage-dependent and repressed by decavanadate. Calmodulin-binding confers the Ca(2+) sensitivity. ATP is able to restore Ca(2+) sensitivity after desensitization. Phosphatidylinositol 4,5-biphosphate (PIP2)-binding strongly enhances activity, by increasing the channel's Ca(2+) sensitivity and shifting its voltage dependence of activation towards negative potentials. Activity is also enhanced by 3,5-bis(trifluoromethyl)pyrazole derivative (BTP2). SUBUNIT: Homomultimer. SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Widely expressed with a high expression in intestine and prostaste. In brain, it is both expressed in whole cerebral arteries and isolated vascular smooth muscle cells. Also known as: Transient receptor potential cation channel subfamily M member 4, long transient receptor potential channel 4, hTRPM4, melastatin-4, calcium-activated non-selective cation channel 1, TRPM4, TRPM4B, FLJ20041.|
|Specificity||Appears to be specific for TRPM4.|
|Application Notes||IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.|
|Storage||Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.|
|Restrictions||For Research Use only|
Xu, Moebius, Gill et al.: "Regulation of melastatin, a TRP-related protein, through interaction with a cytoplasmic isoform." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 19, pp. 10692-7, 2001 (PubMed).
Launay, Fleig, Perraud et al.: "TRPM4 is a Ca2+-activated nonselective cation channel mediating cell membrane depolarization." in: Cell, Vol. 109, Issue 3, pp. 397-407, 2002 (PubMed).
Hofmann, Chubanov, Gudermann et al.: "TRPM5 is a voltage-modulated and Ca(2+)-activated monovalent selective cation channel." in: Current biology : CB, Vol. 13, Issue 13, pp. 1153-8, 2003 (PubMed).
Ota, Suzuki, Nishikawa et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).
Earley, Waldron, Brayden: "Critical role for transient receptor potential channel TRPM4 in myogenic constriction of cerebral arteries." in: Circulation research, Vol. 95, Issue 9, pp. 922-9, 2004 (PubMed).
Launay, Cheng, Srivatsan et al.: "TRPM4 regulates calcium oscillations after T cell activation." in: Science (New York, N.Y.), Vol. 306, Issue 5700, pp. 1374-7, 2004 (PubMed).
Nilius, Prenen, Janssens et al.: "The selectivity filter of the cation channel TRPM4." in: The Journal of biological chemistry, Vol. 280, Issue 24, pp. 22899-906, 2005 (PubMed).
Zhang, Okawa, Wang et al.: "Phosphatidylinositol 4,5-bisphosphate rescues TRPM4 channels from desensitization." in: The Journal of biological chemistry, Vol. 280, Issue 47, pp. 39185-92, 2005 (PubMed).
Takezawa, Cheng, Beck et al.: "A pyrazole derivative potently inhibits lymphocyte Ca2+ influx and cytokine production by facilitating transient receptor potential melastatin 4 channel activity." in: Molecular pharmacology, Vol. 69, Issue 4, pp. 1413-20, 2006 (PubMed).
Cheng, Beck, Launay et al.: "TRPM4 controls insulin secretion in pancreatic beta-cells." in: Cell calcium, Vol. 41, Issue 1, pp. 51-61, 2006 (PubMed).
|Hosts||Rabbit (37), Mouse (4), Goat (1), Sheep (1)|
|Reactivities||Human (30), Mouse (Murine) (23), Rat (Rattus) (21), Cow (Bovine) (1), Dog (Canine) (1)|
|Applications||Western Blotting (WB) (24), Immunohistochemistry (IHC) (17), Immunofluorescence (IF) (15), ELISA (5), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (3), Immunohistochemistry (Paraffin-embedded Sections) pro (IHC (p)+) (3), Immunoelectron Microscopy (IEM) (1), Immunoprecipitation (IP) (1)|
|Conjugates||Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE-Cy3 (1), PE-Cy5 (1), PE-Cy5.5 (1), PE-Cy7 (1), Un-conjugated (1)|
|Epitopes||C-Term (8), N-Term (4), Transcript Variant 1 (4), Internal Region (2), 4th Cytoplasmic Domain (1)|