Transient Receptor Potential Cation Channel, Subfamily M, Member 4 (TRPM4) (C-Term) antibody

Antigen: Transient Receptor Potential Cation Channel, Subfamily M, Member 4 (TRPM4)

Synonyms: TRPM4B, FLJ20041, LTRPC4, LTrpC-4, AW047689, 1110030C19Rik, Mls2s, trpm4, TRPM4

Binding Site: C-Term

Clonality: Polyclonal

Host: Sheep

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN351201

Additional Information

Alternative name: TRPM4 (Melastatin-4)

UniProt: Q8TD43

Immunogen: A synthetic peptide as a part of human transient receptor potential cation channel subfamily M member 4 (TRPM4, melastatin-4) conjugated to immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: FUNCTION: Calcium-activated non selective (CAN) cation channel that mediates membrane depolarization. While it is activated by increase in intracellular Ca(2+), it is impermeable to it. Mediates transport of monovalent cations (Na(+) > K(+) > Cs(+) > Li(+)), leading to depolarize the membrane. It thereby plays a central role in cadiomyocytes, neurons from entorhinal cortex, dorsal root andvomeronasal neurons, endocrine pancreas cells, kidney epithelial cells, cochlea hair cells etc. Participates in T-cell activation by modulating Ca(2+) oscillations after T lymphocyte activation, which is required for NFAT-dependent IL2 production. Involved in myogenic constriction of cerebral arteries. Controls insulin secretion in pancreatic beta-cells. May also be involved in pacemaking or could cause irregular electrical activity under conditions of Ca(2+) overload. ENZYME REGULATION: Gating is voltage-dependent and repressed by decavanadate. Calmodulin-binding confers the Ca(2+) sensitivity. ATP is able to restore Ca(2+) sensitivity after desensitization. Phosphatidylinositol 4,5-biphosphate (PIP2)-binding strongly enhances activity, by increasing the channel's Ca(2+) sensitivity and shifting its voltage dependence of activation towards negative potentials. Activity is also enhanced by 3,5-bis(trifluoromethyl)pyrazole derivative (BTP2). SUBUNIT: Homomultimer. SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Widely expressed with a high expression in intestine and prostaste. In brain, it is both expressed in whole cerebral arteries and isolated vascular smooth muscle cells. Also known as: Transient receptor potential cation channel subfamily M member 4, long transient receptor potential channel 4, hTRPM4, melastatin-4, calcium-activated non-selective cation channel 1, TRPM4, TRPM4B, FLJ20041.

Specificity: Appears to be specific for TRPM4.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Images

A cell cytospin of human lung cancer cell line A549 was fixed with cold acetone for 90 seconds and air-dried. Cells were incubated with the blocking buffer (PBS containing 5% FCS) for 30 minutes at room temperature. Cells were then washed once in PBS and incubated with primary antibody, diluted 1:100 in the blocking buffer, for 30 minutes. Slides were washed 3X in PBS and incubated with Goat anti-sheep conjugated to Alexa-488, diluted 1:200 in blocking buffer, for 30 minutes at room temperature in the dark. Slides were washed as above and mounting media (10% Glycerol in PBS) containing Hoechst 33258 1 µg/ml was used for nuclear counterstaining.  Fluorescent cell staining were analysed using a Olympus microscope and the analysis LS Research software.

Publications

Xu, Moebius, Gill et al.: "Regulation of melastatin, a TRP-related protein, through interaction with a cytoplasmic isoform." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 19, pp. 10692-7, 2001 (PubMed).

Launay, Fleig, Perraud et al.: "TRPM4 is a Ca2+-activated nonselective cation channel mediating cell membrane depolarization." in: Cell, Vol. 109, Issue 3, pp. 397-407, 2002 (PubMed).

Hofmann, Chubanov, Gudermann et al.: "TRPM5 is a voltage-modulated and Ca(2+)-activated monovalent selective cation channel." in: Current biology : CB, Vol. 13, Issue 13, pp. 1153-8, 2003 (PubMed).

Ota, Suzuki, Nishikawa et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).

Earley, Waldron, Brayden: "Critical role for transient receptor potential channel TRPM4 in myogenic constriction of cerebral arteries." in: Circulation research, Vol. 95, Issue 9, pp. 922-9, 2004 (PubMed).

Launay, Cheng, Srivatsan et al.: "TRPM4 regulates calcium oscillations after T cell activation." in: Science (New York, N.Y.), Vol. 306, Issue 5700, pp. 1374-7, 2004 (PubMed).

Nilius, Prenen, Janssens et al.: "The selectivity filter of the cation channel TRPM4." in: The Journal of biological chemistry, Vol. 280, Issue 24, pp. 22899-906, 2005 (PubMed).

Zhang, Okawa, Wang et al.: "Phosphatidylinositol 4,5-bisphosphate rescues TRPM4 channels from desensitization." in: The Journal of biological chemistry, Vol. 280, Issue 47, pp. 39185-92, 2005 (PubMed).

Takezawa, Cheng, Beck et al.: "A pyrazole derivative potently inhibits lymphocyte Ca2+ influx and cytokine production by facilitating transient receptor potential melastatin 4 channel activity." in: Molecular pharmacology, Vol. 69, Issue 4, pp. 1413-20, 2006 (PubMed).

Cheng, Beck, Launay et al.: "TRPM4 controls insulin secretion in pancreatic beta-cells." in: Cell calcium, Vol. 41, Issue 1, pp. 51-61, 2006 (PubMed).