Transient Receptor Potential Cation Channel, Subfamily M, Member 5 (TRPM5) (4th Extracellular Loop) antibody

Antigen: Transient Receptor Potential Cation Channel, Subfamily M, Member 5 (TRPM5)

Synonyms: MTR1, LTRPC5, Mtr1, Ltrpc5, LTrpC-5, 9430099A16Rik

Binding Site: 4th Extracellular Loop

Clonality: Polyclonal

Host: Rabbit

Reactivity: Rat (Rattus)

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN351206

Additional Information

Alternative name: TRPM5 (LTRPC5)

UniProt: 145298

Immunogen: A synthetic peptide from the 4th extracellular loop of rat TRPM5 (LTRPC5, MTR1, Long transient receptor potential channel 5) conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: FUNCTION: Voltage-modulated Ca(2+)-activated, monovalent cation channel (VCAM) that mediates a transient membrane depolarization and plays a central role in taste transduction. Monovalent-specific, non-selective cation channel that mediates the transport of Na(+), K(+) and Cs(+) ions equally well. Activated directly by increases in intracellular Ca(2+), but is impermeable to it. Gating is voltage-dependent and displays rapid activation and deactivation kinetics upon channel stimulation even during sustained elevations in Ca(2+). Also activated by a fast intracellular Ca(2+) increase in response to inositol 1,4,5-triphosphate-producing receptor agonists. The channel is blocked by extracellular acidification. External acidification has 2 effects, a fast reversible block of the current and a slower irreversible enhancement of current inactivation. Is a highly temperature-sensitive, heat activated channel showing a steep increase of inward currents at temperatures between 15 and 35 degrees Celsius. Heat activation is due to a shift of the voltage-dependent activation curve to negative potentials. Activated by arachidonic acid in vitro. May be involved in perception of bitter, sweet and umami tastes. May also be involved in sensing semiochemicals. SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Strongly expressed in fetal brain, liver and kidney, and in adult prostate, testis, ovary, colon and peripheral blood leukocytes. Also expressed in a large proportion of Wilms' tumors and rhabdomyosarcomas. In monochromosomal cell lines shows exclusive paternal expression. Also known as: LTRPC5, MLSN1- and TRP-related gene 1 protein, TRPM5, LTRPC5, MTR1, Transient receptor potential cation channel subfamily M member 5.

Specificity: Appears to be specific for TRPM5.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Nagase, Kikuno, Nakayama et al.: "Prediction of the coding sequences of unidentified human genes. XVIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." in: DNA research : an international journal for rapid publication of reports on genes and genomes, Vol. 7, Issue 4, pp. 273-81, 2000 (PubMed).

Grimm, Kraft, Sauerbruch et al.: "Molecular and functional characterization of the melastatin-related cation channel TRPM3." in: The Journal of biological chemistry, Vol. 278, Issue 24, pp. 21493-501, 2003 (PubMed).

Lee, Chen, Sun et al.: "Expression and characterization of human transient receptor potential melastatin 3 (hTRPM3)." in: The Journal of biological chemistry, Vol. 278, Issue 23, pp. 20890-7, 2003 (PubMed).

Humphray, Oliver, Hunt et al.: "DNA sequence and analysis of human chromosome 9." in: Nature, Vol. 429, Issue 6990, pp. 369-74, 2004 (PubMed).