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TRPM6 antibody (Transient Receptor Potential Cation Channel, Subfamily M, Member 6) (AA 550-600)

Details for Product anti-TRPM6 Antibody No. ABIN351211, Supplier: Log in to see
Antigen
  • CHAK2
  • HMGX
  • HOMG
  • HOMG1
  • HSH
  • TRPM6
  • TRPM6/CHAK2
Epitope
AA 550-600
14
10
3
2
1
1
1
1
1
1
1
Reactivity
Mouse (Murine)
36
19
18
Host
Rabbit
40
2
1
Clonality
Polyclonal
Conjugate
This TRPM6 antibody is un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
26
17
11
11
4
1
Supplier
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Immunogen A synthetic peptide from aa region 550-600 of mouse TRPM6 conjugated to an immunogenic carrier protein was used as the antigen.
Specificity Specific for TRPM6.
Purification Whole serum
Alternative Name TRPM6 (TRPM6 Antibody Abstract)
Background Function: Essential ion channel and serine/threonine-protein kinase. Crucial for magnesium homeostasis. Has an important role in epithelial magnesium transport and in the active magnesium absorption in the gut and kidney. Isoforms of the type M6-kinase lack the ion channel region.
Catalytic activity: ATP + a protein = ADP + a phosphoprotein.
Subunit: Forms heterodimers with TRPM7. TRPM6 requires the presence of TRPM7 to be targeted to the cell membrane (in HEK 293 cells).
Subcellular location: Membrane, Multi-pass membrane protein.
Tissue specificity: Highly expressed in kidney and colon. Isoform TRPM6a and isoform TRPM6b, are coexpressed with TRPM7 in kidney, and testis, and are also found in several cell lines of lung origin. Isoform TRPM6c is detected only in testis and in H510 small cell lung carcinoma cells. Also known as: Transient receptor potential cation channel subfamily M member 6, channel kinase 2, melastatin-related TRP cation channel 6, CHAK2, HSH, HMGX, HOMG, HOMG1.
Research Area Signaling, Metabolism
Application Notes A dilution of 1 : 300 to 1 : 2000 is recommended.
The optimal dilution should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Background publications Hermosura, Nayakanti, Dorovkov, Calderon, Ryazanov, Haymer, Garruto: "A TRPM7 variant shows altered sensitivity to magnesium that may contribute to the pathogenesis of two Guamanian neurodegenerative disorders." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 32, pp. 11510-5, 2005

Chubanov, Waldegger, Mederos y Schnitzler, Vitzthum, Sassen, Seyberth, Konrad, Gudermann: "Disruption of TRPM6/TRPM7 complex formation by a mutation in the TRPM6 gene causes hypomagnesemia with secondary hypocalcemia." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 9, pp. 2894-9, 2004

Dorovkov, Ryazanov: "Phosphorylation of annexin I by TRPM7 channel-kinase." in: The Journal of biological chemistry, Vol. 279, Issue 49, pp. 50643-6, 2004

Ryazanova, Dorovkov, Ansari, Ryazanov: "Characterization of the protein kinase activity of TRPM7/ChaK1, a protein kinase fused to the transient receptor potential ion channel." in: The Journal of biological chemistry, Vol. 279, Issue 5, pp. 3708-16, 2004

Ota, Suzuki, Nishikawa, Otsuki, Sugiyama, Irie, Wakamatsu, Hayashi, Sato, Nagai, Kimura, Makita, Sekine, Obayashi, Nishi, Shibahara, Tanaka, Ishii, Yamamoto, Saito, Kawai, Isono, Nakamura, Nagahari et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs. ..." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003

Schmitz, Perraud, Johnson, Inabe, Smith, Penner, Kurosaki, Fleig, Scharenberg: "Regulation of vertebrate cellular Mg2+ homeostasis by TRPM7." in: Cell, Vol. 114, Issue 2, pp. 191-200, 2003

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