Transient Receptor Potential Cation Channel, Subfamily M, Member 6 (TRPM6) (4th Cytoplasmic Loop) antibody

Antigen: Transient Receptor Potential Cation Channel, Subfamily M, Member 6 (TRPM6)

Synonyms: HSH, HMGX, HOMG, CHAK2, HOMG1, TRPM6/CHAK2, TRPM6

Binding Site: 4th Cytoplasmic Loop

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN351213

Additional Information

Alternative name: TRPM6 (CHAK2, HSH)

UniProt: Q9BX84

Immunogen: A synthetic peptide from the 4th cytoplasmic loop of human TRPM6 (CHAK2, HSH, HMGX, HOMG, HOMG1) conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: FUNCTION: Essential ion channel and serine/threonine-protein kinase. Crucial for magnesium homeostasis. Has an important role in epithelial magnesium transport and in the active magnesium absorption in the gut and kidney. Isoforms of the type M6-kinase lack the ion channel region. CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein. SUBUNIT: Forms heterodimers with TRPM7. TRPM6 requires the presence of TRPM7 to be targeted to the cell membrane (in HEK 293 cells). SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Highly expressed in kidney and colon. Isoform TRPM6a and isoform TRPM6b, are coexpressed with TRPM7 in kidney, and testis, and are also found in several cell lines of lung origin. Isoform TRPM6c is detected only in testis and in H510 small cell lung carcinoma cells. Also known as: Transient receptor potential cation channel subfamily M member 6, channel kinase 2, melastatin-related TRP cation channel 6, CHAK2, HSH, HMGX, HOMG, HOMG1.

Specificity: Appears to be specific for TRPM6.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Riazanova, Pavur, Petrov et al.: "[Novel type of signaling molecules: protein kinases covalently linked to ion channels]" in: Molekuliarnaia biologiia, Vol. 35, Issue 2, pp. 321-32, 2001 (PubMed).

Schlingmann, Weber, Peters et al.: "Hypomagnesemia with secondary hypocalcemia is caused by mutations in TRPM6, a new member of the TRPM gene family." in: Nature genetics, Vol. 31, Issue 2, pp. 166-70, 2002 (PubMed).

Chubanov, Waldegger, Mederos y Schnitzler et al.: "Disruption of TRPM6/TRPM7 complex formation by a mutation in the TRPM6 gene causes hypomagnesemia with secondary hypocalcemia." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 9, pp. 2894-9, 2004 (PubMed).

Humphray, Oliver, Hunt et al.: "DNA sequence and analysis of human chromosome 9." in: Nature, Vol. 429, Issue 6990, pp. 369-74, 2004 (PubMed).