Transient Receptor Potential Cation Channel, Subfamily M, Member 7 (TRPM7) (4th Cytoplasmic Loop) antibody

Details for Product No. ABIN351216
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Antigen
Synonyms cb495, TRPM7, chak, chak1, ltrpc7, trp-plik, ALSPDC, CHAK, CHAK1, LTRPC7, TRP-PLIK, 2310022G15Rik, 4833414K03Rik, 5033407O22Rik, LTrpC-7, Ltpr7, Ltrpc7, TRPPLIK, Chak, Trp-plik
Epitope
4th Cytoplasmic Loop
(43), (22), (11), (7), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity
Human, Rat (Rattus)
(63), (56), (35), (19), (19), (17), (17), (3), (2)
Host
Sheep
(62), (26), (9), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(4), (4), (4), (4), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(75), (33), (30), (29), (24), (21), (19), (14), (2), (1), (1)
Pubmed 6 references available
Quantity 100 μL
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Catalog No. ABIN351216
454.67 $
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Immunogen A synthetic peptide from the 4th cytoplasmic loop of human TRPM7 (LTRPC7) conjugated to an immunogenic carrier protein was used as the antigen.
Specificity Specific for TRPM7.
Purification Whole serum
Alternative Name TRPM7
Background TRPCs, mammalian homologs of the Drosophila transient receptor potential (trp) protein, are ion channels that are thought to mediate capacitative calcium entry into the cell. TRP-PLIK is a protein that is both an ion channel and a kinase. As a channel, it conducts calcium and monovalent cations to depolarize cells and increase intracellular calcium. As a kinase, it is capable of phosphorylating itself and other substrates. The kinase activity is necessary for channel function, as shown by its dependence on intracellular ATP and by the kinase mutants.
Function: Essential ion channel and serine/threonine-protein kinase. Divalent cation channel permeable to calcium and magnesium. Has a central role in magnesium ion homeostasis and in the regulation of anoxic neuronal cell death. The kinase activity is essential for the channel function. May be involved in a fundamental process that adjusts plasma membrane divalent cation fluxes according to the metabolic state of the cell. Phosphorylates annexin A1 (ANXA1).
Catalytic activity: ATP + a protein: ADP + a phosphoprotein.
Cofactor: Binds 1 zinc ion per subunit.
Subunit: Homodimer. Forms heterodimers with TRPM6.
Subcellular location: Membrane, Multi-pass membrane protein. Also known as: Transient receptor potential cation channel subfamily M member 7, long transient receptor potential channel 7, LTrpC7, channel-kinase 1, CHAK1, LTRPC7, CHAK, FLJ20117, FLJ25718, TRP-PLIK.
Research Area Neurology
Application Notes A dilution of 1 : 200 to 1 : 2000 is recommended.
The optimal dilution should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
General Schmitz, Perraud, Johnson et al.: "Regulation of vertebrate cellular Mg2+ homeostasis by TRPM7." in: Cell, Vol. 114, Issue 2, pp. 191-200, 2003 (PubMed).

Ryazanova, Dorovkov, Ansari et al.: "Characterization of the protein kinase activity of TRPM7/ChaK1, a protein kinase fused to the transient receptor potential ion channel." in: The Journal of biological chemistry, Vol. 279, Issue 5, pp. 3708-16, 2004 (PubMed).

Ota, Suzuki, Nishikawa et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs. ..." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).

Chubanov, Waldegger, Mederos y Schnitzler et al.: "Disruption of TRPM6/TRPM7 complex formation by a mutation in the TRPM6 gene causes hypomagnesemia with secondary hypocalcemia." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 9, pp. 2894-9, 2004 (PubMed).

Dorovkov, Ryazanov: "Phosphorylation of annexin I by TRPM7 channel-kinase." in: The Journal of biological chemistry, Vol. 279, Issue 49, pp. 50643-6, 2004 (PubMed).

Hermosura, Nayakanti, Dorovkov et al.: "A TRPM7 variant shows altered sensitivity to magnesium that may contribute to the pathogenesis of two Guamanian neurodegenerative disorders." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 32, pp. 11510-5, 2005 (PubMed).

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