TRPM7 (LTrpC7, Channel-kinase 1,TRP-PLIK, Chak) (4th Cytoplasmic Loop) antibody

Antigen: TRPM7 (LTrpC7, Channel-kinase 1,TRP-PLIK, Chak)

Binding Site: 4th Cytoplasmic Loop

Clonality: Polyclonal

Host: Rabbit

Reactivity: Rat (Rattus)

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN351222

Additional Information

UniProt: Q925B3

Immunogen: A synthetic peptide from the 4th cytoplasmic loop of rat TRPM7 (LTrpC7, Channel-kinase 1, TRP-PLIK, Chak) conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: FUNCTION: Essential ion channel and serine/threonine-protein kinase. Divalent cation channel permeable to calcium and magnesium. Has a central role in magnesium ion homeostasis and in the regulation of anoxic neuronal cell death. The kinase activity is essential for the channel function. May be involved in a fundamental process that adjusts plasma membrane divalent cation fluxes according to the metabolic state of the cell. Phosphorylates annexin A1 (ANXA1). CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein. COFACTOR: Binds 1 zinc ion per subunit. SUBUNIT: Forms heterodimers with TRPM6. Homodimer. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Found to be expressed in brain and skeletal muscle, with stronger signals in kidney, heart, liver, and spleen (at protein level). Also known as: Long transient receptor potential channel 7, LTrpC7, Channel-kinase 1, Transient receptor potential-phospholipase C-interacting kinase, TRP-PLIK, Chak.

Specificity: Appears to be specific for TRPM7.

Application Details

Application Notes: IHC, WB (confirmed by recombinant protein). A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Runnels, Yue, Clapham: "TRP-PLIK, a bifunctional protein with kinase and ion channel activities." in: Science (New York, N.Y.), Vol. 291, Issue 5506, pp. 1043-7, 2001 (PubMed).

Nadler, Hermosura, Inabe et al.: "LTRPC7 is a Mg.ATP-regulated divalent cation channel required for cell viability." in: Nature, Vol. 411, Issue 6837, pp. 590-5, 2001 (PubMed).

Yamaguchi, Matsushita, Nairn et al.: "Crystal structure of the atypical protein kinase domain of a TRP channel with phosphotransferase activity." in: Molecular cell, Vol. 7, Issue 5, pp. 1047-57, 2001 (PubMed).

Carninci, Kasukawa, Katayama et al.: "The transcriptional landscape of the mammalian genome." in: Science (New York, N.Y.), Vol. 309, Issue 5740, pp. 1559-63, 2005 (PubMed).

Villén, Beausoleil, Gerber et al.: "Large-scale phosphorylation analysis of mouse liver." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 5, pp. 1488-93, 2007 (PubMed).