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Transient Receptor Potential Cation Channel, Subfamily M, Member 8 (TRPM8) (4th Cytoplasmic Loop) antibody

Details for Product No. ABIN351224, Supplier: Log in to see
Antigen
  • TRPM8
  • trpp8
  • ltrpc6
  • trpm8b
  • LTRPC6
  • TRPP8
  • CMR1
  • LTrpC-6
  • Trp-p8
Epitope
4th Cytoplasmic Loop
15
13
13
12
9
5
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
Reactivity
Rat (Rattus)
103
44
32
3
3
3
2
2
2
1
1
1
1
1
1
1
1
Host
Sheep
114
14
1
Clonality
Polyclonal
Conjugate
Un-conjugated
5
3
3
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
76
43
38
28
11
7
7
1
Supplier
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Immunogen A synthetic peptide from the 4th cytoplasmic loop of rat TRPM8, conjugated to an immunogenic carrier protein was used as the antigen.
Specificity Specific for TRPM8.
Purification Whole serum
Alternative Name TRPM8 (TRPM8 Antibody Abstract)
Background Function: Receptor-activated non-selective cation channel involved in detection of sensations such as coolness, by being activated by cold temperature below 25 degrees Celsius. Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium. Temperature sensing is tightly linked to voltage-dependent gating. Activated upon depolarization, changes in temperature resulting in graded shifts of its voltage-dependent activation curves. The chemical agonists menthol functions as a gating modifier, shifting activation curves towards physiological membrane potentials. Temperature sensitivity arises from a tenfold difference in the activation energies associated with voltage-dependent opening and closing.
Subcellular location: Membrane, Multi-pass membrane protein (Probable).
Tissue specificity: Expressed in dorsal root and trigeminal ganglia. Specifically expressed in a subset of sensory neurons, including cold-sensitive neurons in trigeminal neurons. SIMILARITY: Belongs to the transient receptor family, LTrpC subfamily. Also known as: Transient receptor potential cation channel subfamily M member 8, Cold menthol receptor 1, Trpm8, Trpm8 transient receptor potential cation channel, subfamily M, member 8, CMR1.
Research Area Neurology, Metabolism
Pathways
Application Notes A dilution of 1 : 300 to 1 : 2000 is recommended.
The optimal dilution should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Background publications Brauchi, Orta, Mascayano et al.: "Dissection of the components for PIP2 activation and thermosensation in TRP channels." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 24, pp. 10246-51, 2007 (PubMed).

Frederick, Buck, Matson et al.: "Increased TRPA1, TRPM8, and TRPV2 expression in dorsal root ganglia by nerve injury." in: Biochemical and biophysical research communications, Vol. 358, Issue 4, pp. 1058-64, 2007 (PubMed).

Abe, Hosokawa, Okazawa et al.: "TRPM8 protein localization in trigeminal ganglion and taste papillae." in: Brain research. Molecular brain research, Vol. 136, Issue 1-2, pp. 91-8, 2005 (PubMed).

Nealen, Gold, Thut et al.: "TRPM8 mRNA is expressed in a subset of cold-responsive trigeminal neurons from rat." in: Journal of neurophysiology, Vol. 90, Issue 1, pp. 515-20, 2003 (PubMed).

McKemy, Neuhausser, Julius: "Identification of a cold receptor reveals a general role for TRP channels in thermosensation." in: Nature, Vol. 416, Issue 6876, pp. 52-8, 2002 (PubMed).

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