Transient Receptor Potential Cation Channel, Subfamily M, Member 8 (TRPM8) (4th cytoplasmic loop) antibody

Antigen: Transient Receptor Potential Cation Channel, Subfamily M, Member 8 (TRPM8)

Synonyms: TRPP8, LTRPC6, MGC2849, CMR1, Trp-p8, LTrpC-6, TRPM8, trpp8, ltrpc6, trpm8b

Epitope: 4th cytoplasmic loop

Clonality: Polyclonal

Host: Sheep

Reactivity: Rat (Rattus)

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN351224

Additional Information

Alternative name: TRPM8 (4th cytoplasmic loop)

Swiss-Prot: Q8R455

Immunogen: A synthetic peptide from the 4th cytoplasmic loop of rat TRPM8, conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: FUNCTION: Receptor-activated non-selective cation channel involved in detection of sensations such as coolness, by being activated by cold temperature below 25 degrees Celsius. Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium. Temperature sensing is tightly linked to voltage-dependent gating. Activated upon depolarization, changes in temperature resulting in graded shifts of its voltage-dependent activation curves. The chemical agonists menthol functions as a gating modifier, shifting activation curves towards physiological membrane potentials. Temperature sensitivity arises from a tenfold difference in the activation energies associated with voltage-dependent opening and closing. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein (Probable). TISSUE SPECIFICITY: Expressed in dorsal root and trigeminal ganglia. Specifically expressed in a subset of sensory neurons, including cold-sensitive neurons in trigeminal neurons. SIMILARITY: Belongs to the transient receptor family, LTrpC subfamily. Also known as: Transient receptor potential cation channel subfamily M member 8, Cold menthol receptor 1, Trpm8, Trpm8 transient receptor potential cation channel, subfamily M, member 8, CMR1.

Specificity: Appears to be specific for TRPM8.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: McKemy, Neuhausser, Julius: "Identification of a cold receptor reveals a general role for TRP channels in thermosensation." in: Nature, Vol. 416, Issue 6876, pp. 52-8, 2002 (PubMed).

Nealen, Gold, Thut et al.: "TRPM8 mRNA is expressed in a subset of cold-responsive trigeminal neurons from rat." in: Journal of neurophysiology, Vol. 90, Issue 1, pp. 515-20, 2003 (PubMed).

Abe, Hosokawa, Okazawa et al.: "TRPM8 protein localization in trigeminal ganglion and taste papillae." in: Brain research. Molecular brain research, Vol. 136, Issue 1-2, pp. 91-8, 2005 (PubMed).

Frederick, Buck, Matson et al.: "Increased TRPA1, TRPM8, and TRPV2 expression in dorsal root ganglia by nerve injury." in: Biochemical and biophysical research communications, Vol. 358, Issue 4, pp. 1058-64, 2007 (PubMed).

Brauchi, Orta, Mascayano et al.: "Dissection of the components for PIP2 activation and thermosensation in TRP channels." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 24, pp. 10246-51, 2007 (PubMed).