Transient Receptor Potential Cation Channel, Subfamily M, Member 8 (TRPM8) (1st Cytoplasmic Loop) antibody

Antigen: Transient Receptor Potential Cation Channel, Subfamily M, Member 8 (TRPM8)

Synonyms: TRPP8, LTRPC6, MGC2849, CMR1, Trp-p8, LTrpC-6, TRPM8, trpp8, ltrpc6, trpm8b

Binding Site: 1st Cytoplasmic Loop

Clonality: Polyclonal

Host: Rabbit

Reactivity: Rat (Rattus), Mouse (Murine)

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN351225

Additional Information

Alternative name: TRPM8 (LTRPC6,TRPP8, LtrpC6)

UniProt: Q8R455

Immunogen: A synthetic peptide from the 1t cytoplasmic loop of rat TRPM8 (LTRPC6, TRPP8, LTrpC6) conjugated to an immunogenic carrie protein was used as the immunogen. The antigen is homologous with the corresponding sequence in mouse.

Format: Lyophilized

Description: FUNCTION: Receptor-activated non-selective cation channel involved in detection of sensations such as coolness, by being activated by cold temperature below 25 degrees Celsius. Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium. Temperature sensing is tightly linked to voltage-dependent gating. Activated upon depolarization, changes in temperature resulting in graded shifts of its voltage-dependent activation curves. The chemical agonists menthol functions as a gating modifier, shifting activation curves towards physiological membrane potentials. Temperature sensitivity arises from a tenfold difference in the activation energies associated with voltage-dependent opening and closing. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Expressed in prostate. Also expressed in most in prostate tumors. Also expressed in non-prostatic primary tumors such as colon, lung, breast and skin tumors. MISCELLANEOUS: The sensation of coolness triggered by eucalyptol or menthol may be explained by the fact that menthol and cool temperatures sensations are detected by this protein. Its expression in most prostate tumors as well as the presence of an immunogenic epitope suggest that it may be suitable for the design of peptide vaccination strategies for prostate cancers. Also known as: Transient receptor potential cation channel subfamily M member 8, transient receptor potential-p8, Trp-p8, long transient receptor potential channel 6, LTrpC6, TRPP8, LTRPC6, MGC2849.

Specificity: Appears to be specific for TRPM8.

Application Details

Application Notes: IHC, WB. A dilution of 1: 300 to 1: 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

McKemy, Neuhausser, Julius: "Identification of a cold receptor reveals a general role for TRP channels in thermosensation." in: Nature, Vol. 416, Issue 6876, pp. 52-8, 2002 (PubMed).

Nealen, Gold, Thut et al.: "TRPM8 mRNA is expressed in a subset of cold-responsive trigeminal neurons from rat." in: Journal of neurophysiology, Vol. 90, Issue 1, pp. 515-20, 2003 (PubMed).