Transient Receptor Potential Cation Channel, Subfamily M, Member 8 (TRPM8) (4th Cytoplasmic Loop) antibody

Details for Product No. ABIN351231
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Antigen
Synonyms TRPM8, trpp8, ltrpc6, trpm8b, LTRPC6, TRPP8, CMR1, LTrpC-6, Trp-p8
Epitope
4th Cytoplasmic Loop
(10), (7), (6), (5), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity
Human
(69), (29), (27), (13), (3), (2), (1), (1)
Host
Rabbit
(81), (3), (2), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(62), (26), (25), (24), (10), (10), (4), (2), (2), (1), (1)
Pubmed 3 references available
Quantity 100 µL
Options
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Catalog No. ABIN351231
454.67 $
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Immunogen A synthetic peptide from the 4th cytoplasmic loop of human TRPM8 (TRPP8) conjugated to an immunogenic carrier protein was used as the antigen. The antigen is homologous in monkey.
Specificity Specific for TRPM8.
Purification Whole serum
Alternative Name TRPM8
Background Function: Receptor-activated non-selective cation channel involved in detection of sensations such as coolness, by being activated by cold temperature below 25 degrees Celsius. Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium. Temperature sensing is tightly linked to voltage-dependent gating. Activated upon depolarization, changes in temperature resulting in graded shifts of its voltage-dependent activation curves. The chemical agonists menthol functions as a gating modifier, shifting activation curves towards physiological membrane potentials. Temperature sensitivity arises from a tenfold difference in the activation energies associated with voltage-dependent opening and closing.
Subcellular location: Membrane, Multi-pass membrane protein.
Tissue specificity: Expressed in prostate. Also expressed in most in prostate tumors. Also expressed in non-prostatic primary tumors such as colon, lung, breast and skin tumors.
Miscellaneous: The sensation of coolness triggered by eucalyptol or menthol may be explained by the fact that menthol and cool temperatures sensations are detected by this protein. Its expression in most prostate tumors as well as the presence of an immunogenic epitope suggest that it may be suitable for the design of peptide vaccination strategies for prostate cancers. Also known as: Transient receptor potential cation channel subfamily M member 8, transient receptor potential-p8, Trp-p8, long transient receptor potential channel 6, LTrpC6, TRPP8, LTRPC6, MGC2849.
Research Area Neurology, Metabolism
Application Notes A dilution of 1: 300 to 1: 2000 is recommended.
The optimal dilution should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
General Tsavaler, Shapero, Morkowski et al.: "Trp-p8, a novel prostate-specific gene, is up-regulated in prostate cancer and other malignancies and shares high homology with transient receptor potential calcium channel proteins." in: Cancer research, Vol. 61, Issue 9, pp. 3760-9, 2001 (PubMed).

Kiessling, Füssel, Schmitz et al.: "Identification of an HLA-A*0201-restricted T-cell epitope derived from the prostate cancer-associated protein trp-p8." in: The Prostate, Vol. 56, Issue 4, pp. 270-9, 2003 (PubMed).

Voets, Droogmans, Wissenbach et al.: "The principle of temperature-dependent gating in cold- and heat-sensitive TRP channels." in: Nature, Vol. 430, Issue 7001, pp. 748-54, 2004 (PubMed).

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