Transient Receptor Potential Cation Channel, Subfamily V, Member 1 (TRPV1) (1st extracellular loop) antibody

Antigen: Transient Receptor Potential Cation Channel, Subfamily V, Member 1 (TRPV1)

Synonyms: VR1, DKFZp434K0220, Vr1, Vr1l1, VR.5'sv, VR-1, OTRPC1, TRPV1beta, TRPV1alpha, TRPV1, Trpv1

Epitope: 1st extracellular loop

Clonality: Polyclonal

Host: Rabbit

Reactivity: Rat (Rattus)

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN351239

Additional Information

Alternative name: TRPV1 (VR1, capsaicin receptor, vanilloid receptor 1) (1t extracellular loop)

Swiss-Prot: O35433

Immunogen: A synthetic peptide from the 1t extracellular loop of rat TRPV1 (VR1, capsaicin receptor, vanilloid receptor 1) conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: FUNCTION: Receptor-activated non-selective calcium permeant cation channel involved in detection of noxious chemical and thermal stimuli. Seems to mediate proton influx and may be involved in intracellular acidosis in nociceptive neurons. May be involved in mediation of inflammatory pain and hyperalgesia. Sensitized by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases, which involves PKC isozymes and PCL. Activation by vanilloids, like capsaicin, and temperatures higher than 42 degrees Celsius, exhibits a time- and Ca(2+)-dependent outward rectification, followed by a long-lasting refractory state. Mild extracellular acidic pH (6.5) potentiates channel activation by noxious heat and vanilloids, whereas acidic conditions (pH Also known as: Transient receptor potential cation channel subfamily V member 1, TrpV1, osm-9-like TRP channel 1, OTRPC1, Vanilloid receptor 1, Vanilloid receptor type 1-like, Capsaicin receptor, transient receptor potential cation channel, subfamily V, member 1, Vr1, Vr1l1, VR.5'sv.

Specificity: Appears to be specific for TRPV1.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Garcuia-Martuinez, Morenilla-Palao, Planells-Cases et al.: "Identification of an aspartic residue in the P-loop of the vanilloid receptor that modulates pore properties." in: The Journal of biological chemistry, Vol. 275, Issue 42, pp. 32552-8, 2000 (PubMed).

Jahnel, Dreger, Gillen et al.: "Biochemical characterization of the vanilloid receptor 1 expressed in a dorsal root ganglia derived cell line." in: European journal of biochemistry / FEBS, Vol. 268, Issue 21, pp. 5489-96, 2001 (PubMed).

Hellwig, Plant, Janson et al.: "TRPV1 acts as proton channel to induce acidification in nociceptive neurons." in: The Journal of biological chemistry, Vol. 279, Issue 33, pp. 34553-61, 2004 (PubMed).

Garcuia-Sanz, Fernuandez-Carvajal, Morenilla-Palao et al.: "Identification of a tetramerization domain in the C terminus of the vanilloid receptor." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 24, Issue 23, pp. 5307-14, 2004 (PubMed).

Wang, Kedei, Wang et al.: "Interaction between protein kinase Cmu and the vanilloid receptor type 1." in: The Journal of biological chemistry, Vol. 279, Issue 51, pp. 53674-82, 2004 (PubMed).