Transient Receptor Potential Cation Channel, Subfamily V, Member 1 (TRPV1) (4th Cytoplasmic Loop) antibody

Antigen: Transient Receptor Potential Cation Channel, Subfamily V, Member 1 (TRPV1)

Synonyms: VR1, DKFZp434K0220, Vr1, Vr1l1, VR.5'sv, VR-1, OTRPC1, TRPV1beta, TRPV1alpha, TRPV1, Trpv1

Binding Site: 4th Cytoplasmic Loop

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN351251

Additional Information

Alternative name: TRPV1 (VR1, capsaicin receptor, vanilloid receptor 1)

UniProt: Q8NER1

Immunogen: A synthetic peptide from the 4th cytoplasmic loop of human TRPV1 (VR1, capsaicin receptor, vanilloid receptor 1) conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: Capsaicin, the main pungent ingredient in hot chili peppers, elicits a sensation of burning pain by selectively activating sensory neurons that convey information about noxious stimuli to the central nervous system. The protein encoded by this gene is a receptor for capsaicin and is a non-selective cation channel that is structurally related to members of the TRP family of ion channels. This receptor is also activated by increases in temperature in the noxious range, suggesting that it functions as a transducer of painful thermal stimuli in vivo. FUNCTION: Receptor-activated non-selective calcium permeant cation channel involved in detection of noxious chemical and thermal stimuli. Seems to mediate proton influx and may be involved in intracellular acidosis in nociceptive neurons. May be involved in mediation of inflammatory pain and hyperalgesia. Sensitized by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases, which involves PKC isozymes and PCL. Responses evoked by low pH and heat, and capsaicin can be antagonized by capsazepine. Also known as: TrpV1, osm-9-like TRP channel 1, OTRPC1, vanilloid receptor 1, capsaicin receptor, VR1, DKFZp434K0220, transient receptor potential cation channel subfamily V member 1.

Specificity: Appears to be specific for TRPV1.

Application Details

Application Notes: IHC, WB. A dilution of 1: 300 to 1: 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Hayes, Meadows, Gunthorpe et al.: "Cloning and functional expression of a human orthologue of rat vanilloid receptor-1." in: Pain, Vol. 88, Issue 2, pp. 205-15, 2001 (PubMed).

McIntyre, McLatchie, Chambers et al.: "Pharmacological differences between the human and rat vanilloid receptor 1 (VR1)." in: British journal of pharmacology, Vol. 132, Issue 5, pp. 1084-94, 2001 (PubMed).

Wiemann, Weil, Wellenreuther et al.: "Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." in: Genome research, Vol. 11, Issue 3, pp. 422-35, 2001 (PubMed).

Cortright, Crandall, Sanchez et al.: "The tissue distribution and functional characterization of human VR1." in: Biochemical and biophysical research communications, Vol. 281, Issue 5, pp. 1183-9, 2001 (PubMed).

Smith, Gunthorpe, Kelsell et al.: "TRPV3 is a temperature-sensitive vanilloid receptor-like protein." in: Nature, Vol. 418, Issue 6894, pp. 186-90, 2002 (PubMed).

Staender, Moormann, Schumacher et al.: "Expression of vanilloid receptor subtype 1 in cutaneous sensory nerve fibers, mast cells, and epithelial cells of appendage structures." in: Experimental dermatology, Vol. 13, Issue 3, pp. 129-39, 2004 (PubMed).

Gavva, Klionsky, Qu et al.: "Molecular determinants of vanilloid sensitivity in TRPV1." in: The Journal of biological chemistry, Vol. 279, Issue 19, pp. 20283-95, 2004 (PubMed).