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|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Transient Receptor Potential Cation Channel, Subfamily V, Member 2 (TRPV2) (3rd Extracellular Loop) antibody
|Synonyms||VRL, VRL1, VRL-1, MGC12549, GRC, Vrl1, OTRPC2, MGC105451, TRPV2|
3rd Extracellular Loop
Alternatives Immunohistochemistry (IHC), Western Blotting (WB)
|6 references available|
|Quantity||100 µl (Variants)|
|Price||454.67 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 7 to 8 Business Days|
|Alternative name||TRPV2 (VRL-1)|
|Immunogen||A synthetic peptide from the 3d extracellular loop of rat TRPV2 (VRL-1) conjugated to an immunogenic carrier protein was used as the immunogen.|
|Description||FUNCTION: Calcium-permeable, non-selective cation channel with an outward rectification. Seems to be regulated, at least in part, by growth factors, like IGF1, PDGF and morphogenetic neuropeptide/head activator. May transduce physical stimuli in mast cells. Activated by temperatures higher than 52 degrees Celsius; is not activated by vanilloids and acidic pH. SUBUNIT: Homotetramer (Probable). Interacts with a cAMP-dependent protein kinase type II regulatory subunit (PRKAR2A or PRKAR2B) and ACBD3. Interacts with RGA. SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Cytoplasm. Melanosome. TISSUE SPECIFICITY: Ubiquitously expressed. Expressed in dorsal root ganglia, trigeminal ganglia, spinal chord (Lissauer's tract, dorsal horn and dorsal columns) (at protein level). PTM: N-glycosylated. PTM: Phosphorylated by PKA. SIMILARITY: Belongs to the transient receptor family, TrpV subfamily. SIMILARITY: Contains 3 ANK repeats. Also known as: Transient receptor potential cation channel subfamily V member 2, TrpV2, osm-9-like TRP channel 2, OTRPC2, Vanilloid receptor-like protein 1, VRL-1, Stretch-activated channel 2B, Sac2b, Vrl1, MGC105451.|
|Specificity||Appears to be specific for TRPV2.|
|Application Notes||IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.|
|Storage||Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.|
|Restrictions||For Research Use only|
Caterina, Rosen, Tominaga et al.: "A capsaicin-receptor homologue with a high threshold for noxious heat." in: Nature, Vol. 398, Issue 6726, pp. 436-41, 1999 (PubMed).
Jung, Lee, Hwang et al.: "Agonist recognition sites in the cytosolic tails of vanilloid receptor 1." in: The Journal of biological chemistry, Vol. 277, Issue 46, pp. 44448-54, 2002 (PubMed).
Zahner, Li, Chen et al.: "Cardiac vanilloid receptor 1-expressing afferent nerves and their role in the cardiogenic sympathetic reflex in rats." in: The Journal of physiology, Vol. 551, Issue Pt 2, pp. 515-23, 2003 (PubMed).
Ichikawa, Sugimoto: "The co-expression of VR1 and VRL-1 in the rat vagal sensory ganglia." in: Brain research, Vol. 980, Issue 2, pp. 293-6, 2003 (PubMed).
Stokes, Shimoda, Koblan-Huberson et al.: "A TRPV2-PKA signaling module for transduction of physical stimuli in mast cells." in: The Journal of experimental medicine, Vol. 200, Issue 2, pp. 137-47, 2004 (PubMed).
Frederick, Buck, Matson et al.: "Increased TRPA1, TRPM8, and TRPV2 expression in dorsal root ganglia by nerve injury." in: Biochemical and biophysical research communications, Vol. 358, Issue 4, pp. 1058-64, 2007 (PubMed).
|Hosts||Rabbit (40), Goat (6), Sheep (2)|
|Reactivities||Mouse (Murine) (26), Rat (Rattus) (26), Human (15)|
|Applications||Western Blotting (WB) (22), Immunofluorescence (IF) (16), Flow Cytometry (FACS) (15), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (13), ELISA (10), Immunohistochemistry (IHC) (10), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Fixed) (IHC (fx)) (1), Immunoprecipitation (IP) (1)|
|Conjugates||Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1)|
|Epitopes||N-Term (6), C-Term (3), 3rd Extracellular Loop (2), 4th Cytoplasmic Loop (2), Internal Region (2), 1st Cytoplasmic Loop (1), 1st Extracellular Loop (1), AA 744-761 (1), N-Term,AA 51-81 (1), Pore-Forming Domain (1)|