Transient Receptor Potential Cation Channel, Subfamily V, Member 4 (TRPV4) (1st Cytoplasmic Domain) antibody

Antigen: Transient Receptor Potential Cation Channel, Subfamily V, Member 4 (TRPV4)

Synonyms: VRL2, TRP12, VRL-2, VROAC, OTRPC4, VR-OAC, TRPV4, wu:fp52e02, Trp12, MGC150050, 0610033B08Rik, Vroac, Otrpc4

Binding Site: 1st Cytoplasmic Domain

Clonality: Polyclonal

Host: Rabbit

Reactivity: Mouse (Murine)

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN351280

Additional Information

Alternative name: TRPV4 (VRL-2)

UniProt: Q9EPK8

Immunogen: A synthetic peptide from 1t cytoplasmic domain of mouse TRPV4 conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: TRPV4 is a cation-selective channel, activated in response to systemic osmotic pressure. FUNCTION: Non-selective calcium permeant cation channel probably involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by low pH, citrate and phorbol esters. Increase of intracellular Ca(2+) potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism. SUBUNIT: Interacts with calmodulin. Interacts with Map7 and Src family Tyr protein kinases LYN, SRC, FYN, HCK, LCK and YES. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Expressed lung, spleen, kidney, testis, fat, and at very low levels in trigeminal ganglia. Also known as: Transient receptor potential cation channel subfamily V member 4, TrpV4, osm-9-like TRP channel 4, OTRPC4, Vanilloid receptor-like channel 2, Vanilloid receptor-like protein 2, Vanilloid receptor-related osmotically-activated channel, VR-OAC, Transient receptor potential protein 12, TRP12, Trp12; VRL-2, VROAC, OTRPC4, VR-OAC, MGC150050, 0610033B08Rik, Trp12, Vrl2, Vroac.

Specificity: Appears to be specific for TRPV4.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Strotmann, Harteneck, Nunnenmacher et al.: "OTRPC4, a nonselective cation channel that confers sensitivity to extracellular osmolarity." in: Nature cell biology, Vol. 2, Issue 10, pp. 695-702, 2000 (PubMed).

Liedtke, Choe, Martui-Renom et al.: "Vanilloid receptor-related osmotically activated channel (VR-OAC), a candidate vertebrate osmoreceptor." in: Cell, Vol. 103, Issue 3, pp. 525-35, 2000 (PubMed).

Wissenbach, Boedding, Freichel et al.: "Trp12, a novel Trp related protein from kidney." in: FEBS letters, Vol. 485, Issue 2-3, pp. 127-34, 2000 (PubMed).

Voets, Prenen, Vriens et al.: "Molecular determinants of permeation through the cation channel TRPV4." in: The Journal of biological chemistry, Vol. 277, Issue 37, pp. 33704-10, 2002 (PubMed).

Xu, Zhao, Tian et al.: "Regulation of a transient receptor potential (TRP) channel by tyrosine phosphorylation. SRC family kinase-dependent tyrosine phosphorylation of TRPV4 on TYR-253 mediates its response to hypotonic stress." in: The Journal of biological chemistry, Vol. 278, Issue 13, pp. 11520-7, 2003 (PubMed).

Suzuki, Mizuno, Kodaira et al.: "Impaired pressure sensation in mice lacking TRPV4." in: The Journal of biological chemistry, Vol. 278, Issue 25, pp. 22664-8, 2003 (PubMed).

Suzuki, Hirao, Mizuno: "Microtubule-associated [corrected] protein 7 increases the membrane expression of transient receptor potential vanilloid 4 (TRPV4)." in: The Journal of biological chemistry, Vol. 278, Issue 51, pp. 51448-53, 2003 (PubMed).