Transient Receptor Potential Cation Channel, Subfamily V, Member 4 (TRPV4) (1t cytoplasmic domain) antibody

Antigen: Transient Receptor Potential Cation Channel, Subfamily V, Member 4 (TRPV4)

Synonyms: VRL2, TRP12, VRL-2, VROAC, OTRPC4, VR-OAC, TRPV4, wu:fp52e02, Trp12, MGC150050, 0610033B08Rik, Vroac, Otrpc4

Epitope: 1t cytoplasmic domain

Clonality: Polyclonal

Host: Sheep

Reactivity: Rat (Rattus)

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN351281

Additional Information

Alternative name: TRPV4 (VRL-2) (1t cytoplasmic domain)

Swiss-Prot: Q9ERZ8

Immunogen: A synthetic peptide from 1t cytoplasmic domain of rat TRPV4 conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: TRPV4 is a cation-selective channel, activated in response to systemic osmotic pressure. FUNCTION: Non-selective calcium permeant cation channel probably involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by low pH, citrate and phorbol esters. Increase of intracellular Ca(2+) potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism. SUBUNIT: Interacts with calmodulin. Interacts with Map7 and Src family Tyr protein kinases LYN, SRC, FYN, HCK, LCK and YES. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Expressed lung, spleen, kidney, testis, fat, and at very low levels in trigeminal ganglia. Also known as: Transient receptor potential cation channel subfamily V member 4, TrpV4, osm-9-like TRP channel 4, OTRPC4, Vanilloid receptor-related, smotically-activated channel, VR-OAC, Vroac, Otrpc4.

Specificity: Appears to be specific for TRPV4.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Liedtke, Choe, Martui-Renom et al.: "Vanilloid receptor-related osmotically activated channel (VR-OAC), a candidate vertebrate osmoreceptor." in: Cell, Vol. 103, Issue 3, pp. 525-35, 2000 (PubMed).

Liedtke, Tobin, Bargmann et al.: "Mammalian TRPV4 (VR-OAC) directs behavioral responses to osmotic and mechanical stimuli in Caenorhabditis elegans." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100 Suppl 2, pp. 14531-6, 2003 (PubMed).

Earley, Heppner, Nelson et al.: "TRPV4 forms a novel Ca2+ signaling complex with ryanodine receptors and BKCa channels." in: Circulation research, Vol. 97, Issue 12, pp. 1270-9, 2005 (PubMed).

Tsushima, Mori: "Antidipsogenic effects of a TRPV4 agonist, 4alpha-phorbol 12,13-didecanoate, injected into the cerebroventricle." in: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 290, Issue 6, pp. R1736-41, 2006 (PubMed).

Koehler, Heyken, Heinau et al.: "Evidence for a functional role of endothelial transient receptor potential V4 in shear stress-induced vasodilatation." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 26, Issue 7, pp. 1495-502, 2006 (PubMed).

Marrelli, Oneil, Brown et al.: "PLA2 and TRPV4 channels regulate endothelial calcium in cerebral arteries." in: American journal of physiology. Heart and circulatory physiology, Vol. 292, Issue 3, pp. H1390-7, 2007 (PubMed).