Transient Receptor Potential Cation Channel, Subfamily V, Member 4 (TRPV4) (1st cytoplasmic loop) antibody

Antigen: Transient Receptor Potential Cation Channel, Subfamily V, Member 4 (TRPV4)

Synonyms: VRL2, TRP12, VRL-2, VROAC, OTRPC4, VR-OAC, TRPV4, wu:fp52e02, Trp12, MGC150050, 0610033B08Rik, Vroac, Otrpc4

Epitope: 1st cytoplasmic loop

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN351282

Additional Information

Alternative name: TRPV4 (VRL-2) (1t cytoplasmic loopl)

Swiss-Prot: Q9EPK8

Immunogen: A synthetic peptide from the 1t cytoplasmic loop of mouse TRPV4 (VRL-2) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in rat and human.

Format: Lyophilized

Description: TRPV4 is a cation-selective channel, activated in response to systemic osmotic pressure. FUNCTION: Non-selective calcium permeant cation channel probably involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by low pH, citrate and phorbol esters. Increase of intracellular Ca(2+) potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism. SUBUNIT: Interacts with calmodulin. Interacts with Map7 and Src family Tyr protein kinases LYN, SRC, FYN, HCK, LCK and YES. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Expressed lung, spleen, kidney, testis, fat, and at very low levels in trigeminal ganglia. SIMILARITY: Belongs to the transient receptor family, TrpV subfamily. SIMILARITY: Contains 3 ANK repeats. Also known as: Transient receptor potential cation channel subfamily V member 4, TrpV4, osm-9-like TRP channel 4, OTRPC4, Vanilloid receptor-like channel 2, Vanilloid receptor-like protein 2, Vanilloid receptor-related osmotically-activated channel, VR-OAC, Transient receptor potential protein 12, TRP12, Trp12; VRL-2, VROAC, OTRPC4, VR-OAC, MGC150050, 0610033B08Rik, Trp12, Vrl2, Vroac.

Specificity: Appears to be specific for TRPV4.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other application.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Images

WB on mouse brain lysate (reduced, denatured) using Rabbit antibody to 1t cytoplasmic loop of TRPV4 (VRL-2): whole serum (ABIN351282) at 1 : 1000 dilution.

Publications

Publications: Strotmann, Harteneck, Nunnenmacher et al.: "OTRPC4, a nonselective cation channel that confers sensitivity to extracellular osmolarity." in: Nature cell biology, Vol. 2, Issue 10, pp. 695-702, 2000 (PubMed).

Liedtke, Choe, Martui-Renom et al.: "Vanilloid receptor-related osmotically activated channel (VR-OAC), a candidate vertebrate osmoreceptor." in: Cell, Vol. 103, Issue 3, pp. 525-35, 2000 (PubMed).

Wissenbach, Boedding, Freichel et al.: "Trp12, a novel Trp related protein from kidney." in: FEBS letters, Vol. 485, Issue 2-3, pp. 127-34, 2000 (PubMed).

Strotmann, Schultz, Plant: "Ca2+-dependent potentiation of the nonselective cation channel TRPV4 is mediated by a C-terminal calmodulin binding site." in: The Journal of biological chemistry, Vol. 278, Issue 29, pp. 26541-9, 2003 (PubMed).

Earley, Heppner, Nelson et al.: "TRPV4 forms a novel Ca2+ signaling complex with ryanodine receptors and BKCa channels." in: Circulation research, Vol. 97, Issue 12, pp. 1270-9, 2005 (PubMed).

Arniges, Fernuandez-Fernuandez, Albrecht et al.: "Human TRPV4 channel splice variants revealed a key role of ankyrin domains in multimerization and trafficking." in: The Journal of biological chemistry, Vol. 281, Issue 3, pp. 1580-6, 2006 (PubMed).