TRPV4 antibody (AA 300-400)

Catalog No. ABIN351285

Product Details anti-TRPV4 Antibody

Target: TRPV4 (Transient Receptor Potential Cation Channel, Subfamily V, Member 4 (TRPV4))

Binding Specificity: AA 300-400

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRPV4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Specificity: Specific for TRPV4.

Cross-Reactivity: Human, Marmoset, Mouse, Rat

Cross-Reactivity (Details): Other species not yet tested.

Purification: IgG

Immunogen: A synthetic peptide from AA 300-400 of mouse TRPV4 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and human.

Isotype: IgG

Application Details

Application Notes: IHC, WB. A concentration of 10-50 μg,ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other application.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.

Handling Advice: Avoid freeze and thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date: 12 months

Target Details for TRPV4

Target: TRPV4 (Transient Receptor Potential Cation Channel, Subfamily V, Member 4 (TRPV4))

Alternative Name: TRPV4 (TRPV4 Products)

Synonyms: wu:fp52e02 antibody, CMT2C antibody, HMSN2C antibody, OTRPC4 antibody, SMAL antibody, SPSMA antibody, SSQTL1 antibody, TRP12 antibody, VRL2 antibody, VROAC antibody, 0610033B08Rik antibody, Trp12 antibody, VR-OAC antibody, VRL-2 antibody, Otrpc4 antibody, Vroac antibody, transient receptor potential cation channel, subfamily V, member 4 antibody, transient receptor potential cation channel subfamily V member 4 antibody, trpv4 antibody, TRPV4 antibody, Trpv4 antibody

Background: TRPV4 is a cation-selective channel, activated in response to systemic osmotic pressure. FUNCTION: Non-selective calcium permeant cation channel probably involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by low pH , citrate and phorbol esters. Increase of intracellular Ca(2+) potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism. SUBUNIT: Interacts with calmodulin. Interacts with Map7 and Src family Tyr protein kinases LYN, SRC, FYN, HCK, LCK and YES. SUBCELLULAR LOCATION: Membrane, Multi-pass membrane protein. TISSUE SPECIFICITY: Expressed lung, spleen, kidney, testis, fat, and at very low levels in trigeminal ganglia. SIMILARITY: Belongs to the transient receptor family, TrpV subfamily. SIMILARITY: Contains 3 ANK repeats.,Mammalian TRPV,Transient receptor potential cation channel subfamily V member 4, TrpV4, osm-9-like TRP channel 4, OTRPC4, Vanilloid receptor-like channel 2, Vanilloid receptor-like protein 2, Vanilloid receptor-related osmotically-activated channel, VR-OAC, Transient receptor potential protein 12, TRP12, Trp12, VRL-2, VROAC, OTRPC4, VR-OAC, MGC150050, 0610033B08Rik, Trp12, Vrl2, Vroac

UniProt: Q9EPK8

Pathways: Hormone Transport, Cell-Cell Junction Organization