Transient Receptor Potential Cation Channel, Subfamily V, Member 5 (TRPV5) (1st Extracellular Domain) antibody

Antigen: Transient Receptor Potential Cation Channel, Subfamily V, Member 5 (TRPV5)

Synonyms: CAT2, ECAC1, OTRPC3, CaT2, Ecac1, MGC129346, MGC129347, D630033B11, TRPV5, ECAC

Epitope: 1st Extracellular Domain

Clonality: Polyclonal

Host: Rabbit

Reactivity: Rat (Rattus)

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN351291

Additional Information

Alternative name: TRPV5 (1t extracellular domain)

Swiss-Prot: Q9JIP0

Immunogen: A synthetic peptide as a part of rat TRPV5 conjugated to immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: FUNCTION: Constitutively active calcium selective cation channel thought to be involved in Ca(2+) reabsorption in kidney and intestine. The channel is activated by low internal calcium level and the current exhibits an inward rectification. A Ca(2+)-dependent feedback regulation includes fast channel inactivation and slow current decay. Heteromeric assembly with TRPV6 seems to modify channel properties. TRPV5-TRPV6 heteromultimeric concatemers exhibit voltage-dependent gating . SUBCELLULAR LOCATION: Apical cell membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Exclusively expressed in kidney. Also known as: Transient receptor potential cation channel subfamily V member 5, TrpV5, osm-9-like TRP channel 3, OTRPC3, Epithelial calcium channel 1, ECaC1, Calcium transporter 2, CaT2, Ecac.

Specificity: Appears to be specific for TRPV5.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Research Area: Signaling, Metabolism

Restrictions: For Research Use only

Publications

Publications: Peng, Chen, Berger et al.: "A rat kidney-specific calcium transporter in the distal nephron." in: The Journal of biological chemistry, Vol. 275, Issue 36, pp. 28186-94, 2000 (PubMed).

van Abel, Hoenderop, van der Kemp et al.: "Coordinated control of renal Ca(2+) transport proteins by parathyroid hormone." in: Kidney international, Vol. 68, Issue 4, pp. 1708-21, 2005 (PubMed).