Transient Receptor Potential Cation Channel, Subfamily V, Member 5 (TRPV5) antibody

Antigen: Transient Receptor Potential Cation Channel, Subfamily V, Member 5 (TRPV5)

Synonyms: CAT2, ECAC1, OTRPC3, CaT2, Ecac1, MGC129346, MGC129347, D630033B11, TRPV5, ECAC

Clonality: Polyclonal

Host: Rabbit

Reactivity: Mouse (Murine)

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN351292

Additional Information

Alternative name: TRPV5

UniProt: P69744

Immunogen: A synthetic peptide as a part of mouse TRPV5 conjugated to immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: FUNCTION: Constitutively active calcium selective cation channel thought to be involved in Ca(2+) reabsorption in kidney and intestine. The channel is activated by low internal calcium level and the current exhibits an inward rectification. A Ca(2+)-dependent feedback regulation includes fast channel inactivation and slow current decay. Heteromeric assembly with TRPV6 seems to modify channel properties. TRPV5-TRPV6 heteromultimeric concatemers exhibit voltage-dependent gating. SUBCELLULAR LOCATION: Apical cell membrane; Multi-pass membrane protein. Note=Colocalized with S100A10 and ANAX2 along the apical domain of kidney distal tubular cells. Also known as: Transient receptor potential cation channel subfamily V member 5, TrpV5, osm-9-like TRP channel 3, OTRPC3, Epithelial calcium channel 1, ECaC1, Calcium transporter 2, CaT2, Ecac,CAT2, ECAC1, MGC129346, MGC129347, D630033B11.

Specificity: Appears to be specific for TRPV5.

Application Details

Application Notes: IHC, WB. A dilution of 1: 300 to 1: 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Research Area: Signaling, Metabolism

Restrictions: For Research Use only

Publications

Nilius, Prenen, Hoenderop et al.: "Fast and slow inactivation kinetics of the Ca2+ channels ECaC1 and ECaC2 (TRPV5 and TRPV6). Role of the intracellular loop located between transmembrane segments 2 and 3." in: The Journal of biological chemistry, Vol. 277, Issue 34, pp. 30852-8, 2002 (PubMed).

van de Graaf, Hoenderop, Gkika et al.: "Functional expression of the epithelial Ca(2+) channels (TRPV5 and TRPV6) requires association of the S100A10-annexin 2 complex." in: The EMBO journal, Vol. 22, Issue 7, pp. 1478-87, 2003 (PubMed).

Lambers, Weidema, Nilius et al.: "Regulation of the mouse epithelial Ca2(+) channel TRPV6 by the Ca(2+)-sensor calmodulin." in: The Journal of biological chemistry, Vol. 279, Issue 28, pp. 28855-61, 2004 (PubMed).

Carninci, Kasukawa, Katayama et al.: "The transcriptional landscape of the mammalian genome." in: Science (New York, N.Y.), Vol. 309, Issue 5740, pp. 1559-63, 2005 (PubMed).

van de Graaf, van der Kemp, van den Berg et al.: "Identification of BSPRY as a novel auxiliary protein inhibiting TRPV5 activity." in: Journal of the American Society of Nephrology : JASN, Vol. 17, Issue 1, pp. 26-30, 2005 (PubMed).

van Abel, Huybers, Hoenderop et al.: "Age-dependent alterations in Ca2+ homeostasis: role of TRPV5 and TRPV6." in: American journal of physiology. Renal physiology, Vol. 291, Issue 6, pp. F1177-83, 2006 (PubMed).

Nakaya, Harbidge, Wangemann et al.: "Lack of pendrin HCO3- transport elevates vestibular endolymphatic [Ca2+] by inhibition of acid-sensitive TRPV5 and TRPV6 channels." in: American journal of physiology. Renal physiology, Vol. 292, Issue 5, pp. F1314-21, 2007 (PubMed).

Topala, Bindels, Hoenderop: "Regulation of the epithelial calcium channel TRPV5 by extracellular factors." in: Current opinion in nephrology and hypertension, Vol. 16, Issue 4, pp. 319-24, 2007 (PubMed).