Transient Receptor Potential Cation Channel, Subfamily V, Member 5 (TRPV5) antibody

Antigen: Transient Receptor Potential Cation Channel, Subfamily V, Member 5 (TRPV5)

Synonyms: CAT2, ECAC1, OTRPC3, CaT2, Ecac1, MGC129346, MGC129347, D630033B11, TRPV5, ECAC

Clonality: Polyclonal

Host: Sheep

Reactivity: Human

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN351295

Additional Information

Alternative name: TRPV5

Swiss-Prot: Q9NQA5

Immunogen: A synthetic peptide fromf human TRPV5 conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: This gene is a member of the transient receptor family and the TrpV subfamily. The calcium-selective channel encoded by this gene has 6transmembrane-spanning domains, multiple potential phosphorylation sites, an N-linked glycosylation site, and 5 ANK repeats. This protein forms homotetramers or heterotetramers and is activated by a low internal calcium level. FUNCTION: Constitutively active calcium selective cation channel thought to be involved in Ca(2+) reabsorption in kidney and intestine. The channel is activated by low internal calcium level and the current exhibits an inward rectification. A Ca(2+)-dependent feedback regulation includes fast channel inactivation and slow current decay. Heteromeric assembly with TRPV6 seems to modify channel properties. TRPV5-TRPV6 heteromultimeric concatemers exhibit voltage-dependent gating. SUBCELLULAR LOCATION: Apical cell membrane; Multi-pass membrane protein. Note=Colocalized with S100A10 and ANAX2 along the apical domain of kidney distal tubular cells. TISSUE SPECIFICITY: Expressed at high levels in kidney, small intestine and pancreas, and at lower levels in testis, prostate, placenta, brain, colon and rectum. Also known as: TrpV5, osm-9-like TRP channel 3, OTRPC3, Epithelial calcium channel 1, ECaC, ECaC1, Calcium transport protein 2, CaT2, CAT2, ECAC1, OTRPC3, transient receptor potential cation channel subfamily V member 5.

Specificity: Appears to be specific for TRPV5.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Research Area: Signaling, Metabolism

Restrictions: For Research Use only

Publications

Publications: Mueller, Hoenderop, Meij et al.: "Molecular cloning, tissue distribution, and chromosomal mapping of the human epithelial Ca2+ channel (ECAC1)." in: Genomics, Vol. 67, Issue 1, pp. 48-53, 2000 (PubMed).

Peng, Brown, Hediger: "Structural conservation of the genes encoding CaT1, CaT2, and related cation channels." in: Genomics, Vol. 76, Issue 1-3, pp. 99-109, 2001 (PubMed).

Vassilev, Peng, Hediger et al.: "Single-channel activities of the human epithelial Ca2+ transport proteins CaT1 and CaT2." in: The Journal of membrane biology, Vol. 184, Issue 2, pp. 113-20, 2001 (PubMed).

Jiang, Ferguson, Peng: "WNK4 enhances TRPV5-mediated calcium transport: potential role in hypercalciuria of familial hyperkalemic hypertension caused by gene mutation of WNK4." in: American journal of physiology. Renal physiology, Vol. 292, Issue 2, pp. F545-54, 2007 (PubMed).

Schoeber, Hoenderop, Bindels: "Concerted action of associated proteins in the regulation of TRPV5 and TRPV6." in: Biochemical Society transactions, Vol. 35, Issue Pt 1, pp. 115-9, 2007 (PubMed).