Transient Receptor Potential Cation Channel, Subfamily V, Member 5 (TRPV5) (4th cytoplasmic loop) antibody

Antigen: Transient Receptor Potential Cation Channel, Subfamily V, Member 5 (TRPV5)

Synonyms: CAT2, ECAC1, OTRPC3, CaT2, Ecac1, MGC129346, MGC129347, D630033B11, TRPV5, ECAC

Epitope: 4th cytoplasmic loop

Clonality: Polyclonal

Host: Rabbit

Reactivity: Dog (Canine)

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN351300

Additional Information

Alternative name: TRPV5 (OTRPC3, Calcium transporter 2, Osm-9-likeTRP channel 3) (4th cytoplasmic loop)

Swiss-Prot: 73978776

Immunogen: A synthetic peptide from the 4th cytoplasmic loop of dog TRPV5 (OTRPC3, Calcium transporter 2, Osm-9-like TRP channel 3) conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: FUNCTION: Constitutively active calcium selective cation channel thought to be involved in Ca(2+) reabsorption in kidney and intestine. The channel is activated by low internal calcium level and the current exhibits an inward rectification. A Ca(2+)-dependent feedback regulation includes fast channel inactivation and slow current decay. Heteromeric assembly with TRPV6 seems to modify channel properties. TRPV5-TRPV6 heteromultimeric concatemers exhibit voltage-dependent gating. SUBUNIT: Homotetramer and probably heterotetramer with TRPV6. Interacts with TRPV6. Interacts with S100A10 and probably with the ANAX2-S100A10 heterotetramer. The interaction with S100A10 is required for the trafficking to the plasma membrane. Interacts with calmodulin. Interacts with BSPRY, which results in its inactivation. SUBCELLULAR LOCATION: Apical cell membrane; Multi-pass membrane protein. Note=Colocalized with S100A10 and ANAX2 along the apical domain of kidney distal tubular cells. SIMILARITY: Belongs to the transient receptor family, TrpV subfamily. SIMILARITY: Contains 4 ANK repeats. Also known as: Transient receptor potential cation channel subfamily V member 5, TrpV5, osm-9-like TRP channel 3, OTRPC3, Epithelial calcium channel 1, ECaC1, Calcium transporter 2, CaT2, Ecac,CAT2, ECAC1.

Specificity: Appears to be specific for TRPV5.

Application Details

Application Notes: IHC, WB. A dilution of 1: 300 to 1: 3000 is recommended. The optimal dilution should be determined by the end user.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Hoenderop, van der Kemp, Hartog et al.: "Molecular identification of the apical Ca2+ channel in 1, 25-dihydroxyvitamin D3-responsive epithelia." in: The Journal of biological chemistry, Vol. 274, Issue 13, pp. 8375-8, 1999 (PubMed).

Nilius, Vennekens, Prenen et al.: "The single pore residue Asp542 determines Ca2+ permeation and Mg2+ block of the epithelial Ca2+ channel." in: The Journal of biological chemistry, Vol. 276, Issue 2, pp. 1020-5, 2001 (PubMed).

Hoenderop, Voets, Hoefs et al.: "Homo- and heterotetrameric architecture of the epithelial Ca2+ channels TRPV5 and TRPV6." in: The EMBO journal, Vol. 22, Issue 4, pp. 776-85, 2003 (PubMed).