Transient Receptor Potential Cation Channel, Subfamily V, Member 6 (TRPV6) (4th cytoplasmic loop) antibody

Antigen: Transient Receptor Potential Cation Channel, Subfamily V, Member 6 (TRPV6)

Synonyms: CAT1, CATL, ZFAB, ECAC2, ABP/ZF, LP6728, HSA277909, CAT, Cac, CaT1, Ecac2, Otrpc3, TRPV6, trpv6, CaT, ECaC, ecac, MGC162545

Epitope: 4th cytoplasmic loop

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN351309

Additional Information

Alternative name: TRPV6 (4th cytoplasmic loop)

Swiss-Prot: Q9H1D0

Immunogen: A synthetic peptide from the 4th cytoplasmic loop of human TRPV6 conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: Calcium-permeable channels, such as TRPV6, participate in neurotransmission, muscle contraction, and exocytosis by providing calcium as an intracellular second messenger. Depending on the tissue, transcellular calcium transport may be regulated by vitamin D, parathyroid hormone (PTH), or calcitonin (CALCA) FUNCTION: Calcium selective cation channel probably involved in Ca(2+) uptake in various tissues, including Ca(2+) reabsorption in intestine. The channel is activated by low internal calcium level, probably including intracellular calcium store depletion, and the current exhibits an inward rectification. Inactivation includes both, a rapid Ca(2+)-dependent and a slower Ca(2+)-calmodulin-dependent mechanism, the latter may be regulated by phosphorylation. In vitro, is slowly inhibited by Mg(2+) in a voltage-independent manner. Heteromeric assembly with TRPV5 seems to modify channel properties. TRPV5-TRPV6 heteromultimeric concatemers exhibit voltage-dependent gating. SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Also known as: TrpV6, epithelial calcium channel 2, ECaC2, calcium transport protein 1, CaT1, CaT-like, CaT-L.

Specificity: Appears to be specific for TRPV6.

Application Details

Application Notes: IHC, WB. A dilution of 1: 300 to 1: 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Peng, Chen, Berger et al.: "Human calcium transport protein CaT1." in: Biochemical and biophysical research communications, Vol. 278, Issue 2, pp. 326-32, 2000 (PubMed).

Niemeyer, Bergs, Wissenbach et al.: "Competitive regulation of CaT-like-mediated Ca2+ entry by protein kinase C and calmodulin." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 6, pp. 3600-5, 2001 (PubMed).

Wissenbach, Niemeyer, Fixemer et al.: "Expression of CaT-like, a novel calcium-selective channel, correlates with the malignancy of prostate cancer." in: The Journal of biological chemistry, Vol. 276, Issue 22, pp. 19461-8, 2001 (PubMed).

Wood, Tchack, Taparia: "1,25-Dihydroxyvitamin D3 increases the expression of the CaT1 epithelial calcium channel in the Caco-2 human intestinal cell line." in: BMC physiology, Vol. 1, pp. 11, 2003 (PubMed).

Peng, Brown, Hediger: "Structural conservation of the genes encoding CaT1, CaT2, and related cation channels." in: Genomics, Vol. 76, Issue 1-3, pp. 99-109, 2001 (PubMed).

Boedding, Flockerzi: "Ca2+ dependence of the Ca2+-selective TRPV6 channel." in: The Journal of biological chemistry, Vol. 279, Issue 35, pp. 36546-52, 2004 (PubMed).