Phosphoprotein (V5 tag) antibody

Antigen: Phosphoprotein

Epitope: V5 tag

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN351320

Additional Information

Alternative name: V5 tag (V5 epitope tag)

Swiss-Prot: P11207

Immunogen: Synthetic peptide {GKPIPNPLLGLDST (95-108)} from Simian virus 5 (strain W3) (SV5) conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: Epitope tag commonly fused with the protein of interest and expressed as a single chain. Also known as: V5 epitope tag, V5 tag, Protein Rev antibody, GKPIPNPLLGLDST epitope tag, Non-structural protein V, Simian virus 5 (strain W3) SV5.

Specificity: Appears to be specific for V5 (GKPIPNPLLGLDST).

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Thomas, Lamb, Paterson: "Two mRNAs that differ by two nontemplated nucleotides encode the amino coterminal proteins P and V of the paramyxovirus SV5." in: Cell, Vol. 54, Issue 6, pp. 891-902, 1988 (PubMed).

Randall, Bermingham: "NP:P and NP:V interactions of the paramyxovirus simian virus 5 examined using a novel protein:protein capture assay." in: Virology, Vol. 224, Issue 1, pp. 121-9, 1996 (PubMed).

Lin, Horvath, Aligo et al.: "The role of simian virus 5 V protein on viral RNA synthesis." in: Virology, Vol. 338, Issue 2, pp. 270-80, 2005 (PubMed).

Precious, Childs, Fitzpatrick-Swallow et al.: "Simian virus 5 V protein acts as an adaptor, linking DDB1 to STAT2, to facilitate the ubiquitination of STAT1." in: Journal of virology, Vol. 79, Issue 21, pp. 13434-41, 2005 (PubMed).

Li, Chen, Garbutt et al.: "Structure of DDB1 in complex with a paramyxovirus V protein: viral hijack of a propeller cluster in ubiquitin ligase." in: Cell, Vol. 124, Issue 1, pp. 105-17, 2006 (PubMed).