Vacuolar Protein Sorting 25 Homolog (S. Cerevisiae) (VPS25) (Internal Region) antibody

Antigen: Vacuolar Protein Sorting 25 Homolog (S. Cerevisiae) (VPS25)

Synonyms: DERP9, EAP20, FAP20, MGC10540, AW107467, D11Wsu68e, 1110020N13Rik, VPS25, dVps25, l(2)44Db, l(2)k08904, DmelCG14750, CG14750, vps25, MGC162560, im:6898134, MGC86224

Binding Site: Internal Region

Clonality: Polyclonal

Host: Rabbit

Reactivity: Please enquire

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN351322

Additional Information

Alternative name: Vacuolar protein sorting-associated protein 25 (VPS25)

UniProt: Q9CQ80

Immunogen: A synthetic peptide from the internal part of mouse Vacuolar protein sorting-associated protein 25 (VPS25) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in rat, zebra fish, xenopus and human.

Format: Lyophilized

Description: FUNCTION: Component of the ESCRT-II complex, which is required for multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery oftransmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL. SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note: Distributes diffusely throughout the cytoplasm and nucleoplasm, but exhibits a punctate distribution on coexpression with CHMP6. Also known as: VPS25.

Specificity: Appears to be specific for VPS25.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Kamura, Burian, Khalili et al.: "Cloning and characterization of ELL-associated proteins EAP45 and EAP20. a role for yeast EAP-like proteins in regulation of gene expression by glucose." in: The Journal of biological chemistry, Vol. 276, Issue 19, pp. 16528-33, 2001 (PubMed).

von Schwedler, Stuchell, Mueller et al.: "The protein network of HIV budding." in: Cell, Vol. 114, Issue 6, pp. 701-13, 2003 (PubMed).

Gibbs, Weinstock, Metzker et al.: "Genome sequence of the Brown Norway rat yields insights into mammalian evolution." in: Nature, Vol. 428, Issue 6982, pp. 493-521, 2004 (PubMed).

Yorikawa, Shibata, Waguri et al.: "Human CHMP6, a myristoylated ESCRT-III protein, interacts directly with an ESCRT-II component EAP20 and regulates endosomal cargo sorting." in: The Biochemical journal, Vol. 387, Issue Pt 1, pp. 17-26, 2005 (PubMed).

Carninci, Kasukawa, Katayama et al.: "The transcriptional landscape of the mammalian genome." in: Science (New York, N.Y.), Vol. 309, Issue 5740, pp. 1559-63, 2005 (PubMed).