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Vacuolar Protein Sorting 25 Homolog (S. Cerevisiae) (VPS25) (Internal Region) antibody

Details for Product No. ABIN351322
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Antigen
Synonyms
DERP9, EAP20, FAP20, AW107467, D11Wsu68e, 1110020N13Rik, VPS25, CG14750, Dmel\\CG14750, ESCRT-I, ESCRT-II, dVps25, l(2)44Db, l(2)k08904, vps25, im:6898134, zgc:162560, GB16268, derp9, eap20, fap20, DD ... show more
DERP9, EAP20, FAP20, AW107467, D11Wsu68e, 1110020N13Rik, VPS25, CG14750, Dmel\\CG14750, ESCRT-I, ESCRT-II, dVps25, l(2)44Db, l(2)k08904, vps25, im:6898134, zgc:162560, GB16268, derp9, eap20, fap20, DDBDRAFT_0189483, DDBDRAFT_0234026, DDB_0189483, DDB_0234026 show less
Epitope
Internal Region
(10), (9), (3), (3), (2)
Reactivity
Zebrafish
(25), (6), (5)
Host
Rabbit
(20), (5), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(25), (17), (6), (5), (1), (1)
Pubmed 5 references available
Quantity 100 µL
Options
Shipping to United States (Change)
Availability Will be delivered in 7 to 8 Business Days
Catalog No. ABIN351322
454.67 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen A synthetic peptide from the internal region of mouse VPS25 conjugated to an immunogenic carrier protein was used as the antigen. The peptide is homologous in rat, zebra fish, xenopus and human.
Isotype IgG
Specificity Specific for TRPV1.
Purification whole serum
Alternative Name Vacuolar protein sorting-associated protein 25 (VPS25)
Background Function: Component of the ESCRT-II complex, which is required for multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery oftransmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation, possibly via its interaction with ELL.
Subcellular location: Cytoplasm. Nucleus. Note: Distributes diffusely throughout the cytoplasm and nucleoplasm, but exhibits a punctate distribution on coexpression with CHMP6. Also known as: VPS25.
UniProt B0S711
Application Notes A dilution of 1 : 300 to 1 : 2000 is recommended.
The optimal dilution should be determined by the end user.
Restrictions For Research Use only
Format Liquid
Reconstitution Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage -20 °C
Storage Comment Maintain the reconstituted antibodies frozen at -20 °C for long term storage and refrigerated at 2-8 °C for a shorter term.
Expiry Date 12 months
General Kamura, Burian, Khalili et al.: "Cloning and characterization of ELL-associated proteins EAP45 and EAP20. a role for yeast EAP-like proteins in regulation of gene expression by glucose." in: The Journal of biological chemistry, Vol. 276, Issue 19, pp. 16528-33, 2001 (PubMed).

von Schwedler, Stuchell, Mueller et al.: "The protein network of HIV budding." in: Cell, Vol. 114, Issue 6, pp. 701-13, 2003 (PubMed).

Gibbs, Weinstock, Metzker et al.: "Genome sequence of the Brown Norway rat yields insights into mammalian evolution." in: Nature, Vol. 428, Issue 6982, pp. 493-521, 2004 (PubMed).

Yorikawa, Shibata, Waguri et al.: "Human CHMP6, a myristoylated ESCRT-III protein, interacts directly with an ESCRT-II component EAP20 and regulates endosomal cargo sorting." in: The Biochemical journal, Vol. 387, Issue Pt 1, pp. 17-26, 2005 (PubMed).

Carninci, Kasukawa, Katayama et al.: "The transcriptional landscape of the mammalian genome." in: Science (New York, N.Y.), Vol. 309, Issue 5740, pp. 1559-63, 2005 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN351322
454.67 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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