Vacuolar Protein Sorting 28 Homolog (S. Cerevisiae) (VPS28) (internal) antibody

Antigen: Vacuolar Protein Sorting 28 Homolog (S. Cerevisiae) (VPS28)

Synonyms: MGC60323, AI847241, 1110014J03Rik, D730005C08Rik, MGC188842, MGC68926, MGC66078, zgc:66078, VPS28, MGC127104, DmVps28, dVps28, l(2)k16503, vps28, DmelCG12770, CG12770, VPL13, VPT28

Epitope: internal

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN351323

Additional Information

Alternative name: Vacuolar protein sorting-associated protein 28 (ASK1, VPS28, CIIA) (internal part)

Swiss-Prot: B5DEN9

Immunogen: A synthetic peptide from the internal part of rat Vacuolar protein sorting-associated protein 28 (ASK1, VPS28, CIIA) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in mouse, human, zebra fish and xenopus.

Format: Lyophilized

Description: This gene encodes a protein involved in endosomal sorting of cell surface receptors via a multivesicular body/late endosome pathway. The encoded protein is one of the three subunits of the ESCRT-I complex (endosomal complexes required for transport) involved in the sorting of ubiquitinated proteins. The two other subunits of ESCRT-I are vesicular protein sorting 23, also known as tumor susceptibility gene 101 (TSG101), and vesicular protein sorting 37. Also known as: ASK1, Caspase-activated DNase inhibitor that interacts with ASK1, VPS28, CIIA.

Specificity: Appears to be specific for VPS28.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Cho, Kim, Lee et al.: "Identification of a novel antiapoptotic protein that antagonizes ASK1 and CAD activities." in: The Journal of cell biology, Vol. 163, Issue 1, pp. 71-81, 2003 (PubMed).

Carninci, Kasukawa, Katayama et al.: "The transcriptional landscape of the mammalian genome." in: Science (New York, N.Y.), Vol. 309, Issue 5740, pp. 1559-63, 2005 (PubMed).