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Vacuolar Protein Sorting 29 Homolog (S. Cerevisiae) (VPS29) (C-Term) antibody

Details for Product No. ABIN351325, Supplier: Log in to see
Antigen
  • VPS29
  • PEP11
  • VPT6
  • DDBDRAFT_0188107
  • DDBDRAFT_0234192
  • DDB_0188107
  • DDB_0234192
  • 17.m07782
  • vps29
  • 2010015D08Rik
  • AW049835
  • fb06g05
  • wu:fb06g05
  • zgc:56191
  • zgc:86638
  • DC15
  • ATVPS29
  • MAIGO 1
  • VACUOLAR PROTEIN SORTING 29
Epitope
C-Term
18
16
2
1
1
1
1
Reactivity
Mouse (Murine), Rat (Rattus), Human
42
13
10
7
4
2
2
2
2
2
Host
Rabbit
34
8
1
Clonality
Polyclonal
Conjugate
Un-conjugated
3
2
2
2
2
2
Application
Immunohistochemistry (IHC), Western Blotting (WB)
30
23
15
6
2
2
Supplier
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Immunogen A synthetic peptide from the c-terminal of mouse VPS29 conjugated to an immunogenic carrier protein was used as the antigen. The antigen is homologous in rat, human, chicken, zebra fish and xenopus.
Specificity Specific for VPS29.
Purification Whole serum
Alternative Name VPS29 (VPS29 Antibody Abstract)
Background Function: Essential component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Also required to regulate transcytosis of the polymeric immunoglobulin receptor (pIgR-pIgA). Has low protein phosphatase activity towards a serine-phosphorylated peptide derived from IGF2R (in vitro). Has no activity towards p-nitrophenylphosphate, p-nitrophenylphosphorylcholine or phosphatidylinositlphosphates (in vitro).
Catalytic activity: O-phospho-L(or D)-serine + H2O = L(or D)-serine + phosphate.
Cofactor: Binds 2 zinc ions per subunit.
Subcellular location: Cytoplasm. Membrane, Peripheral membrane protein. Endosome membrane, Peripheral membrane protein. Also known as: Vacuolar protein sorting-associated protein 29, Vesicle protein sorting 29, VPS29.
Research Area Signaling
Application Notes A dilution of 1 : 300 to 1 : 2000 is recommended.
The optimal dilution should be determined by the end user. Not tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Background publications Hierro, Rojas, Rojas et al.: "Functional architecture of the retromer cargo-recognition complex." in: Nature, Vol. 449, Issue 7165, pp. 1063-7, 2007 (PubMed).

Carninci, Kasukawa, Katayama et al.: "The transcriptional landscape of the mammalian genome. ..." in: Science (New York, N.Y.), Vol. 309, Issue 5740, pp. 1559-63, 2005 (PubMed).

Collins, Skinner, Watson et al.: "Vps29 has a phosphoesterase fold that acts as a protein interaction scaffold for retromer assembly." in: Nature structural & molecular biology, Vol. 12, Issue 7, pp. 594-602, 2005 (PubMed).

Vergues, Luton, Gruber et al.: "The mammalian retromer regulates transcytosis of the polymeric immunoglobulin receptor." in: Nature cell biology, Vol. 6, Issue 8, pp. 763-9, 2004 (PubMed).

Wiemann, Weil, Wellenreuther et al.: "Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs. ..." in: Genome research, Vol. 11, Issue 3, pp. 422-35, 2001 (PubMed).

Haft, de la Luz Sierra, Bafford et al.: "Human orthologs of yeast vacuolar protein sorting proteins Vps26, 29, and 35: assembly into multimeric complexes." in: Molecular biology of the cell, Vol. 11, Issue 12, pp. 4105-16, 2000 (PubMed).