Vacuolar Protein Sorting 45 Homolog (S. Cerevisiae) (VPS45) (internal) antibody

Antigen: Vacuolar Protein Sorting 45 Homolog (S. Cerevisiae) (VPS45)

Synonyms: CG8228, DmelCG8228, dVps45, MENE (3R)-C, MENE(3R)-C, VPS45, Vps45p, ATVPS45, T14N5.2, T14N5_2, VACUOLAR PROTEIN SORTING 45, Vps45, H1, VSP45, VPS45A, VPS45B, VPS54A, VSP45A, H1VPS45, mVps45, AI462172, AW554165, Vsp45a, MGC93104, MGC139503, si:dkey-200h23.3, vps45, MGC146275

Epitope: internal

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN351329

Additional Information

Alternative name: Vacuolar protein sorting-associated protein 45 (rvps45, Vps45, Vps45a) (internal part)

Swiss-Prot: O08700

Immunogen: A synthetic peptide from the internal part of rat Vacuolar protein sorting-associated protein 45 (rvps45, Vps45, Vps45a) conjugated to an immunogenic carrier protein was used as the immunogen. The immunogen is homologous with the corresponding sequence in mouse.

Format: Lyophilized

Isotype: IgG  (Matching secondary antibodies)

Description: FUNCTION: May play a role in vesicle-mediated protein trafficking from the Golgi stack through the trans-Golgi network. SUBUNIT: Interacts with STX6 and ZFYVE20. SUBCELLULAR LOCATION: Golgi apparatus membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein. Note: Associated with Golgi/endosomal vesicles and the trans-Golgi network. TISSUE SPECIFICITY: Ubiquitous; expression was highest in testis and in brain. Detected in every part of the brain. Also known as: Vps45, VPS45, rvps45, Vps45a.

Specificity: Appears to be specific for VPS45.

Synonyms: CG8228, DmelCG8228, dVps45, MENE (3R)-C, MENE(3R)-C, VPS45, Vps45p, ATVPS45, T14N5.2, T14N5_2, VACUOLAR PROTEIN SORTING 45, Vps45, H1, VSP45, VPS45A, VPS45B, VPS54A, VSP45A, H1VPS45, mVps45, AI462172, AW554165, Vsp45a, MGC93104, MGC139503, si:dkey-200h23.3, vps45, MGC146275

Application Details

Application Notes: IHC, WB. A concentration of 10-50 µg/ml is recommended. The optimal concentration should be determined by the end user.

Purity: IgG

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Images

IHC on rat spinal cord using Rabbit antibody to internal part of Vacuolar protein sorting-associated protein 45 (rvps45, Vps45, Vps45a): IgG (ABIN351329) at a concentration of 10 µg/ml. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).

IHC on rat spinal cord using Rabbit antibody to internal part of Vacuolar protein sorting-associated protein 45 (rvps45, Vps45, Vps45a): IgG (ABIN351329) at a concentration of 10 µg/ml. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).

IHC on rat spinal cord using Rabbit antibody to internal part of Vacuolar protein sorting-associated protein 45 (rvps45, Vps45, Vps45a): IgG (ABIN351329) at a concentration of 10 µg/ml. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).

Publications

Publications: Tellam, James, Stevens et al.: "Identification of a mammalian Golgi Sec1p-like protein, mVps45." in: The Journal of biological chemistry, Vol. 272, Issue 10, pp. 6187-93, 1997 (PubMed).

El-Husseini, Guthrie, Snutch et al.: "Molecular cloning of a mammalian homologue of the yeast vesicular transport protein vps45." in: Biochimica et biophysica acta, Vol. 1325, Issue 1, pp. 8-12, 1997 (PubMed).