SNF8, ESCRT-II Complex Subunit, Homolog (S. Cerevisiae) (SNF8) (Internal Region) antibody

Details for Product No. ABIN351332
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Antigen
Synonyms d11moh34, vps22, Dot3, EAP30, VPS22, D11moh34, RGD1310144, D11Moh34, D11MOH34, zgc:101578
Epitope
Internal Region
(16), (8), (7), (4), (2), (2), (2), (2), (2), (1), (1)
Reactivity
Mouse (Murine), Rat (Rattus), Human
(41), (12), (9), (4), (4), (4), (4), (2), (1), (1)
Host
Rabbit
(30), (11), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (2), (2), (2)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(41), (18), (7), (3), (2)
Pubmed 4 references available
Quantity 100 μL
Options
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Catalog No. ABIN351332
454.67 $
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Immunogen A synthetic peptide from the internal region of human SNF8 conjugated to an immunogenic carrier protein was used as the antigen. The antigen is homologous in rat, mouse and xenopus.
Specificity Specific for SNF8.
Purification Whole serum
Alternative Name SNF8
Background Function: Component of the ESCRT-II complex, which is required for multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery oftransmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation by participating in derepression of transcription by RNA polymerase II, possibly via its interaction with ELL.
Subcellular location: Cytoplasm. Nucleus. Also known as: EAP30, SNF8, ELL-associated protein of 30 kDa, ESCRT-II complex subunit VPS22.
Application Notes A dilution of 1 : 300 to 1 : 2000 is recommended.
The optimal dilution should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Supplier Images
anti-SNF8, ESCRT-II Complex Subunit, Homolog (S. Cerevisiae) (SNF8) (Internal Region) antibody WB on rat brain lysate using Rabbit antibody to internal part of Vacuolar sorting protein SNF8: whole serum (ABIN351332) at 1 : 1000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the detected band.
General Schmidt, Miller, Schmidt et al.: "Cloning and characterization of the EAP30 subunit of the ELL complex that confers derepression of transcription by RNA polymerase II." in: The Journal of biological chemistry, Vol. 274, Issue 31, pp. 21981-5, 1999 (PubMed).

Kamura, Burian, Khalili et al.: "Cloning and characterization of ELL-associated proteins EAP45 and EAP20. a role for yeast EAP-like proteins in regulation of gene expression by glucose." in: The Journal of biological chemistry, Vol. 276, Issue 19, pp. 16528-33, 2001 (PubMed).

von Schwedler, Stuchell, Mueller et al.: "The protein network of HIV budding." in: Cell, Vol. 114, Issue 6, pp. 701-13, 2003 (PubMed).

Carninci, Kasukawa, Katayama et al.: "The transcriptional landscape of the mammalian genome. ..." in: Science (New York, N.Y.), Vol. 309, Issue 5740, pp. 1559-63, 2005 (PubMed).

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