SNF8, ESCRT-II Complex Subunit, Homolog (S. Cerevisiae) (SNF8) (internal) antibody

Antigen: SNF8, ESCRT-II Complex Subunit, Homolog (S. Cerevisiae) (SNF8)

Synonyms: Dot3, EAP30, VPS22, D11moh34, MGC105799, RGD1310144, D11MOH34, d11moh34, SNF8, MGC142498, MGC7335, D11Moh34, RP23-235J5.2, zgc:101578, vps22

Epitope: internal

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN351332

Additional Information

Alternative name: Vacuolar sorting protein SNF8 (internal region)

Swiss-Prot: Q96H20

Immunogen: A synthetic peptide from the internal region of human Vacuolar-sorting protein SNF8 conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in rat, mouse and xenopus.

Format: Lyophilized

Description: FUNCTION: Component of the ESCRT-II complex, which is required for multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery oftransmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. The ESCRT-II complex may also play a role in transcription regulation by participating in derepression of transcription by RNA polymerase II, possibly via its interaction with ELL. SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Also known as: EAP30, SNF8, ELL-associated protein of 30 kDa, ESCRT-II complex subunit VPS22.

Specificity: Appears to be specific for SNF8.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Images

WB on rat brain lysate using Rabbit antibody to internal part of Vacuolar sorting protein SNF8: whole serum (ABIN351332) at 1 : 1000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the detected band.

Publications

Publications: Schmidt, Miller, Schmidt et al.: "Cloning and characterization of the EAP30 subunit of the ELL complex that confers derepression of transcription by RNA polymerase II." in: The Journal of biological chemistry, Vol. 274, Issue 31, pp. 21981-5, 1999 (PubMed).

Kamura, Burian, Khalili et al.: "Cloning and characterization of ELL-associated proteins EAP45 and EAP20. a role for yeast EAP-like proteins in regulation of gene expression by glucose." in: The Journal of biological chemistry, Vol. 276, Issue 19, pp. 16528-33, 2001 (PubMed).

von Schwedler, Stuchell, Mueller et al.: "The protein network of HIV budding." in: Cell, Vol. 114, Issue 6, pp. 701-13, 2003 (PubMed).

Carninci, Kasukawa, Katayama et al.: "The transcriptional landscape of the mammalian genome." in: Science (New York, N.Y.), Vol. 309, Issue 5740, pp. 1559-63, 2005 (PubMed).