Vesicle-Associated Membrane Protein 1 (VAMP1) antibody

Antigen: Vesicle-Associated Membrane Protein 1 (VAMP1)

Synonyms: SYB1, VAMP-1, DKFZp686H12131, Syb1, lew, Syb-1, vamp1, MGC75977, zgc:73378, wu:fj59a03

Clonality: Polyclonal

Host: Sheep

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN351335

Additional Information

Alternative name: Vesicle associated membrane protein 1 (VAMP-1, VAMP1, Synaptobrevin-1)

Swiss-Prot: Q63666

Immunogen: A synthetic peptide from rat Vesicle-associated membrane protein 1 (VAMP-1, Vamp1, Synaptobrevin-1, Syb1) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in mouse.

Format: Lyophilized

Isotype: IgG  (Matching secondary antibodies)

Description: FUNCTION: Involved in the targeting and/or fusion of transport vesicles to their target membrane. SUBUNIT: Interacts with VAPA and VAPB. SUBCELLULAR LOCATION: Isoform 1: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Single-pass type IV membrane protein. Cell junction, synapse, synaptosome. SUBCELLULAR LOCATION: Isoform 2: Cytoplasmic vesicle membrane; Single-pass type IV membrane protein. Note: Isoforms 2 and 3 are found in perinuclear as well as peripheral punctate structures in osteoblasts. SUBCELLULAR LOCATION: Isoform 3: Mitochondrion outer membrane; Single-pass type IV membrane protein. Note=Isoforms 2 and 3 are found in perinuclear as well as peripheral punctate structures in osteoblasts. TISSUE SPECIFICITY: Expressed in brain and spleen (at protein level). Isoform 1 expressed at very high level in brain. Even higher level found in spinal cord. Isoform 3 expressed in kidney, spleen and liver. Isoforms 2 and 3 expressed in osteoblasts of trabecular bone. Also expressed in heart. Also known as: VAMP-1, Vamp1, Synaptobrevin-1, Syb1.

Specificity: Appears to be specific for VAMP1.

Application Details

Application Notes: IHC, WB. A concentration of 10-50 µg/ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.

Purity: IgG

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Elferink, Trimble, Scheller: "Two vesicle-associated membrane protein genes are differentially expressed in the rat central nervous system." in: The Journal of biological chemistry, Vol. 264, Issue 19, pp. 11061-4, 1989 (PubMed).

Mandic, Trimble, Lowe: "Tissue-specific alternative RNA splicing of rat vesicle-associated membrane protein-1 (VAMP-1)." in: Gene, Vol. 199, Issue 1-2, pp. 173-9, 1997 (PubMed).

Pruele, Horton, Caterina et al.: "Identification of the molecular mechanisms contributing to polarized trafficking in osteoblasts." in: Experimental cell research, Vol. 282, Issue 1, pp. 24-34, 2002 (PubMed).

Vosseller, Hansen, Chalkley et al.: "Quantitative analysis of both protein expression and serine / threonine post-translational modifications through stable isotope labeling with dithiothreitol." in: Proteomics, Vol. 5, Issue 2, pp. 388-98, 2005 (PubMed).

Carninci, Kasukawa, Katayama et al.: "The transcriptional landscape of the mammalian genome." in: Science (New York, N.Y.), Vol. 309, Issue 5740, pp. 1559-63, 2005 (PubMed).