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Vesicle-Associated Membrane Protein 1 (VAMP1) antibody

Antigen

Vesicle-Associated Membrane Protein 1 (VAMP1)

Synonyms SYB1, VAMP-1, DKFZp686H12131, Syb1, lew, Syb-1, vamp1, MGC75977, zgc:73378, wu:fj59a03
Clonality Polyclonal
Host
Alternatives

Sheep

Application
Alternatives Immunohistochemistry (IHC), Western Blotting (WB)
5 references available
Catalog no. ABIN351335
Quantity 500 µg
Price 413.33 $   Plus shipping costs $35.00
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Additional Information

Alternative name Vesicle associated membrane protein 1 (VAMP-1, VAMP1, Synaptobrevin-1)
Swiss-Prot Q63666
Immunogen A synthetic peptide from rat Vesicle-associated membrane protein 1 (VAMP-1, Vamp1, Synaptobrevin-1, Syb1) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in mouse.
Format Lyophilized
Isotype IgG  (Matching secondary antibodies)
Description FUNCTION: Involved in the targeting and/or fusion of transport vesicles to their target membrane. SUBUNIT: Interacts with VAPA and VAPB. SUBCELLULAR LOCATION: Isoform 1: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Single-pass type IV membrane protein. Cell junction, synapse, synaptosome. SUBCELLULAR LOCATION: Isoform 2: Cytoplasmic vesicle membrane; Single-pass type IV membrane protein. Note: Isoforms 2 and 3 are found in perinuclear as well as peripheral punctate structures in osteoblasts. SUBCELLULAR LOCATION: Isoform 3: Mitochondrion outer membrane; Single-pass type IV membrane protein. Note=Isoforms 2 and 3 are found in perinuclear as well as peripheral punctate structures in osteoblasts. TISSUE SPECIFICITY: Expressed in brain and spleen (at protein level). Isoform 1 expressed at very high level in brain. Even higher level found in spinal cord. Isoform 3 expressed in kidney, spleen and liver. Isoforms 2 and 3 expressed in osteoblasts of trabecular bone. Also expressed in heart. Also known as: VAMP-1, Vamp1, Synaptobrevin-1, Syb1.
Specificity Appears to be specific for VAMP1.

Application Details

Application Notes IHC, WB. A concentration of 10-50 µg/ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.
Purity IgG
Storage Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Restrictions For Research Use only

Publications

Publications Elferink, Trimble, Scheller: "Two vesicle-associated membrane protein genes are differentially expressed in the rat central nervous system." in: The Journal of biological chemistry, Vol. 264, Issue 19, pp. 11061-4, 1989 (PubMed).

Mandic, Trimble, Lowe: "Tissue-specific alternative RNA splicing of rat vesicle-associated membrane protein-1 (VAMP-1)." in: Gene, Vol. 199, Issue 1-2, pp. 173-9, 1997 (PubMed).

Pruele, Horton, Caterina et al.: "Identification of the molecular mechanisms contributing to polarized trafficking in osteoblasts." in: Experimental cell research, Vol. 282, Issue 1, pp. 24-34, 2002 (PubMed).

Vosseller, Hansen, Chalkley et al.: "Quantitative analysis of both protein expression and serine / threonine post-translational modifications through stable isotope labeling with dithiothreitol." in: Proteomics, Vol. 5, Issue 2, pp. 388-98, 2005 (PubMed).

Carninci, Kasukawa, Katayama et al.: "The transcriptional landscape of the mammalian genome." in: Science (New York, N.Y.), Vol. 309, Issue 5740, pp. 1559-63, 2005 (PubMed).

Alternatives

Alternatives for antigen "Vesicle-Associated Membrane Protein 1 (VAMP1)", type "Antibodies"
Hosts Mouse (4), Rabbit (3), Goat (1), Sheep (1)
Reactivities Human (6), Mouse (Murine) (3), Pig (Porcine) (2), Rat (Rattus) (2), Cow (Bovine) (1), Dog (Canine) (1), Hamster (1), Xenopus laevis (1)
Applications Western Blotting (WB) (8), Enzyme Immunoassay (EIA) (4), Immunohistochemistry (IHC) (3), Immunofluorescence (IF) (2), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (2), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1), Immunoprecipitation (IP) (1)