Vesicle-Associated Membrane Protein 4 (VAMP4) (N-Term) antibody

Details for Product No. ABIN351337
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Antigen
Synonyms zgc:73166, zgc:86855, VAMP4, vamp4, VAMP-4, VAMP24, D1Ertd147e
Epitope
N-Term
(12), (12), (9), (7), (2), (2), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(43), (15), (4)
Host
Rabbit
(43), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (2), (2), (2)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(39), (29), (14), (11), (1), (1), (1)
Pubmed 5 references available
Quantity 100 μL
Options
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Catalog No. ABIN351337
454.67 $
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Immunogen A synthetic peptide from the n-terminal of mouse VAMP4 conjugated to an immunogenic carrier protein was used as the antigen. The peptide is homologous in human.
Specificity Specific for VAMP4.
Purification Whole serum
Alternative Name VAMP4
Background Function: Involved in the pathway that functions to remove an inhibitor (probably synaptotagmin-4) of calcium-triggered exocytosis during the maturation of secretory granules. May be a marker for this sorting pathway that is critical for remodeling the secretory response of granule.
Subcellular location: Golgi apparatus › trans-Golgi network membrane, Single-pass type IV membrane protein, associated with trans Golgi network (TGN) and newly formed immature secretory granules (ISG). Not found on the mature secretory organelles. Also known as: VAMP4, Vesicle associated membrane protein 4.
Research Area Signaling
Application Notes A dilution of 1 : 300 to 1 : 2000 is recommended.
The optimal dilution should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
General Ballif, Villén, Beausoleil et al.: "Phosphoproteomic analysis of the developing mouse brain." in: Molecular & cellular proteomics : MCP, Vol. 3, Issue 11, pp. 1093-101, 2004 (PubMed).

Carninci, Kasukawa, Katayama et al.: "The transcriptional landscape of the mammalian genome. ..." in: Science (New York, N.Y.), Vol. 309, Issue 5740, pp. 1559-63, 2005 (PubMed).

Zwilling, Cypionka, Pohl et al.: "Early endosomal SNAREs form a structurally conserved SNARE complex and fuse liposomes with multiple topologies." in: The EMBO journal, Vol. 26, Issue 1, pp. 9-18, 2007 (PubMed).

Dai, Jin, Sheng et al.: "Protein phosphorylation and expression profiling by Yin-yang multidimensional liquid chromatography (Yin-yang MDLC) mass spectrometry." in: Journal of proteome research, Vol. 6, Issue 1, pp. 250-62, 2007 (PubMed).

Zhou, Ye, Dong et al.: "Specific phosphopeptide enrichment with immobilized titanium ion affinity chromatography adsorbent for phosphoproteome analysis." in: Journal of proteome research, Vol. 7, Issue 9, pp. 3957-67, 2008 (PubMed).

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