Vesicle-Associated Membrane Protein 4 (VAMP4) (N-Term) antibody

Antigen: Vesicle-Associated Membrane Protein 4 (VAMP4)

Synonyms: VAMP24, D1Ertd147e, MGC73166, zgc:73166, zgc:86855, VAMP4, vamp4

Epitope: N-Term

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN351337

Additional Information

Alternative name: Vesicle associated membrane protein 4 (VAMP-4, VAMP4) (n-terminal)

Swiss-Prot: O70480

Immunogen: A synthetic peptide from the n-terminal of mouse Vesicle associated membrane protein 4 (VAMP-4, VAMP4) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in human.

Format: Lyophilized

Description: FUNCTION: Involved in the pathway that functions to remove an inhibitor (probably synaptotagmin-4) of calcium-triggered exocytosis during the maturation of secretory granules. May be a marker for this sorting pathway that is critical for remodeling the secretory response of granule. Subcellular location: Golgi apparatus › trans-Golgi network membrane; Single-pass type IV membrane protein; associated with trans Golgi network (TGN) and newly formed immature secretory granules (ISG). Not found on the mature secretory organelles. Also known as: VAMP4, Vesicle associated membrane protein 4.

Specificity: Appears to be specific for VAMP4.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Ballif, Villuen, Beausoleil et al.: "Phosphoproteomic analysis of the developing mouse brain." in: Molecular & cellular proteomics : MCP, Vol. 3, Issue 11, pp. 1093-101, 2004 (PubMed).

Carninci, Kasukawa, Katayama et al.: "The transcriptional landscape of the mammalian genome." in: Science (New York, N.Y.), Vol. 309, Issue 5740, pp. 1559-63, 2005 (PubMed).

Zwilling, Cypionka, Pohl et al.: "Early endosomal SNAREs form a structurally conserved SNARE complex and fuse liposomes with multiple topologies." in: The EMBO journal, Vol. 26, Issue 1, pp. 9-18, 2007 (PubMed).

Dai, Jin, Sheng et al.: "Protein phosphorylation and expression profiling by Yin-yang multidimensional liquid chromatography (Yin-yang MDLC) mass spectrometry." in: Journal of proteome research, Vol. 6, Issue 1, pp. 250-62, 2007 (PubMed).

Zhou, Ye, Dong et al.: "Specific phosphopeptide enrichment with immobilized titanium ion affinity chromatography adsorbent for phosphoproteome analysis." in: Journal of proteome research, Vol. 7, Issue 9, pp. 3957-67, 2008 (PubMed).