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USO1 Homolog, Vesicle Docking Protein (USO1) (Internal Region) antibody

Details for Product No. ABIN351346, Supplier: Log in to see
Antigen
  • MGC80010
  • VDP
  • DKFZp469N0425
  • 115kDa
  • TAP
  • Vdp
  • P115
Epitope
Internal Region
33
15
13
6
2
2
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
83
23
23
Host
Rabbit
60
15
5
3
Clonality
Polyclonal
Conjugate
Un-conjugated
4
4
4
3
3
3
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
66
44
31
11
9
6
1
1
1
Supplier
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Immunogen A synthetic peptide from the internal region of human Vesicle-docking protein (VDP, USO1, TAP, Transcytosis-associated protein) conjugated to an immunogenic carrier protein was used as the antigen. The antigen is homologous in rat and moue.
Isotype IgG
Specificity Specific for VDP.
Alternative Name VDP (USO1 Antibody Abstract)
Background Function: General vesicular transport factor required for intercisternal transport in the Golgi stack, it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity.
Subcellular location: Cytoplasm, cytosol. Golgi apparatus membrane, Peripheral membrane protein. Note: Recycles between the cytosol and the Golgi apparatus during interphase. Also known as: Protein USO1 homolog, Transcytosis-associated protein, TAP, General vesicular transport factor p115, USO1, VDP.
Application Notes A concentration of 10-50 µg/ml is recommended.
The optimal concentration should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Background publications Han, Ye, Zhou et al.: "Large-scale phosphoproteome analysis of human liver tissue by enrichment and fractionation of phosphopeptides with strong anion exchange chromatography." in: Proteomics, Vol. 8, Issue 7, pp. 1346-61, 2008 (PubMed).

Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).

Sohda, Misumi, Yano et al.: "Phosphorylation of the vesicle docking protein p115 regulates its association with the Golgi membrane." in: The Journal of biological chemistry, Vol. 273, Issue 9, pp. 5385-8, 1998 (PubMed).