Vesicle docking protein (VDP, USO1, TAP, Transcytosis-associated protein) (Internal Region) antibody

Antigen: Vesicle docking protein (VDP, USO1, TAP, Transcytosis-associated protein)

Binding Site: Internal Region

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN351346

Additional Information

UniProt: O60763

Immunogen: A synthetic peptide from the internal part of human Vesicle-docking protein (VDP, USO1, TAP, Transcytosis-associated protein) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in rat and moue.

Format: Lyophilized

Isotype: IgG

Description: FUNCTION: General vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity. SUBCELLULAR LOCATION: Cytoplasm, cytosol. Golgi apparatus membrane; Peripheral membrane protein. Note: Recycles between the cytosol and the Golgi apparatus during interphase. Also known as: Protein USO1 homolog, Transcytosis-associated protein, TAP, General vesicular transport factor p115, USO1, VDP.

Specificity: Appears to be specific for VDP.

Application Details

Application Notes: IHC, WB. A concentration of 10-50 µg/ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.

Purity: IgG

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Sohda, Misumi, Yano et al.: "Phosphorylation of the vesicle docking protein p115 regulates its association with the Golgi membrane." in: The Journal of biological chemistry, Vol. 273, Issue 9, pp. 5385-8, 1998 (PubMed).

Olsen, Blagoev, Gnad et al.: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006 (PubMed).

Han, Ye, Zhou et al.: "Large-scale phosphoproteome analysis of human liver tissue by enrichment and fractionation of phosphopeptides with strong anion exchange chromatography." in: Proteomics, Vol. 8, Issue 7, pp. 1346-61, 2008 (PubMed).