Vesicle-Associated Membrane Protein 1 (Synaptobrevin 1) (VAMP1) (AA 2-35) antibody

Details for Product No. ABIN351354
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms SYB1, VAMP-1, syb1, vamp-1, Syb1, lew, Syb-1, VAMP1, An12g07570
Epitope
AA 2-35
(6), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity
Human
(50), (25), (21), (17), (16), (13), (12), (12), (5), (1), (1)
Host
Rabbit
(37), (27), (2), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(51), (23), (19), (10), (9), (8), (6), (1)
Pubmed 5 references available
Catalog no. ABIN351354
Quantity 100 µL
Price
454.67 $   Plus shipping costs $45.00
Options
Shipping to United States (Change)
Availability Will be delivered in 7 to 8 Business Days

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen A synthetic peptide from AA 2-35 of mouse VAMP-1 conjugated to an immunogenic carrier protein was used as the antigen.
Specificity Specific for olfactory receptor OR2T1.
Purification whole serum
Alternative Name Vesicle-associated membrane protein 1 (VAMP-1, Vamp1, Synaptobrevin-1, Syb1)
Background Function: Involved in the targeting and/or fusion of transport vesicles to their target membrane.
Subunit: Interacts with VAPA and VAPB.
Subcellular location: Isoform 1: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane, Single-pass type IV membrane protein. Cell junction, synapse, synaptosome.
Subcellular location: Isoform 2: Cytoplasmic vesicle membrane, Single-pass type IV membrane protein. Note: Isoforms 2 and 3 are found in perinuclear as well as peripheral punctate structures in osteoblasts.
Subcellular location: Isoform 3: Mitochondrion outer membrane, Single-pass type IV membrane protein. Note=Isoforms 2 and 3 are found in perinuclear as well as peripheral punctate structures in osteoblasts.
Tissue specificity: Expressed in brain and spleen (at protein level). Isoform 1 expressed at very high level in brain. Even higher level found in spinal cord. Isoform 3 expressed in kidney, spleen and liver. Isoforms 2 and 3 expressed in osteoblasts of trabecular bone. Also expressed in heart. Also known as: VAMP-1, Vamp1, Synaptobrevin-1, Syb1.
UniProt O43869
Application Notes A dilution of 1 : 300 to 1 : 2000 is recommended.
The optimal dilution should be determined by the end user.
Restrictions For Research Use only
Format Liquid
Reconstitution Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage -20 °C
Storage Comment Maintain the reconstituted antibodies frozen at -20 °C for long term storage and refrigerated at 2-8 °C for a shorter term.
Expiry Date 12 months
Supplier Images
anti-Vesicle-Associated Membrane Protein 1 (Synaptobrevin 1) (VAMP1) (AA 2-35) antibody WB on rat brain lysate using Rabbit antibody to Vesicle-associated membrane protein 1 (VAMP-1, Vamp1, Synaptobrevin-1, Syb1): whole serum (ABIN351354) at 1 : 1000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the detected bands.
General Elferink, Trimble, Scheller: "Two vesicle-associated membrane protein genes are differentially expressed in the rat central nervous system." in: The Journal of biological chemistry, Vol. 264, Issue 19, pp. 11061-4, 1989 (PubMed).

Mandic, Trimble, Lowe: "Tissue-specific alternative RNA splicing of rat vesicle-associated membrane protein-1 (VAMP-1)." in: Gene, Vol. 199, Issue 1-2, pp. 173-9, 1997 (PubMed).

Pruele, Horton, Caterina et al.: "Identification of the molecular mechanisms contributing to polarized trafficking in osteoblasts." in: Experimental cell research, Vol. 282, Issue 1, pp. 24-34, 2002 (PubMed).

Vosseller, Hansen, Chalkley et al.: "Quantitative analysis of both protein expression and serine / threonine post-translational modifications through stable isotope labeling with dithiothreitol." in: Proteomics, Vol. 5, Issue 2, pp. 388-98, 2005 (PubMed).

Carninci, Kasukawa, Katayama et al.: "The transcriptional landscape of the mammalian genome...." in: Science (New York, N.Y.), Vol. 309, Issue 5740, pp. 1559-63, 2005 (PubMed).

Hosts (37), (27), (2), (1)
Reactivities (50), (25), (21), (17), (16), (13), (12), (12), (5), (1), (1)
Applications (51), (23), (19), (10), (9), (8), (6), (1)
Conjugates (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (6), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
back to top