Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2) antibody

Details for Product No. ABIN351357
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Antigen
Synonyms SYB2, VAMP-2, Syb-2, Syb2, sybII, SYB, RATVAMPB, RATVAMPIR, syb2, vamp2, xsybii, zgc:73302, wu:fj61c06, VAMP2, vamp2-a, xsybi
Reactivity
Mouse (Murine)
(62), (32), (24), (12), (12), (12), (12), (12), (1), (1), (1), (1), (1), (1)
Host
Rabbit
(34), (33), (2), (1), (1), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(49), (30), (15), (10), (10), (10), (4), (3), (2), (2), (2), (1)
Pubmed 7 references available
Catalog no. ABIN351357
Quantity 100 µL
Price
454.67 $   Plus shipping costs $45.00
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Immunogen A synthetic peptide from human VAMP2 conjugated to an immunogenic carrier protein was used as the antigen. The peptide is homologous in many other species including rat, mouse and xenopus.
Specificity Specific for MOR22-1.
Purification whole serum
Alternative Name Vesicle-associated membrane protein 2 (VAMP-2, Synaptobrevin-2, VAMP2, SYB2)
Background The protein encoded by this gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated proteinSNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. This gene is thought to participate in neurotransmitter release at a step between docking and fusion. The protein forms a stable complex with syntaxin, synaptosomal-associated protein, 25 kD, and synaptotagmin. It also forms a distinct complex with synaptophysin. It is a likely candidate gene for familial infantile myasthenia (FIMG) because of its map location and because it encodes a synaptic vesicle protein of the type that has been implicated in the pathogenesis of FIMG.
Function: Involved in the targeting and/or fusion of transport vesicles to their target membrane.
Subcellular location: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane, Single-pass type IV membrane protein. Cell junction, synapse, synaptosome. Note: Neuronal synaptic vesicles. Also known as: VAMP-2, Synaptobrevin-2, VAMP2, SYB2.
UniProt Q9EQQ5
Application Notes A dilution of 1 : 300 to 1 : 2000 is recommended.
The optimal dilution should be determined by the end user.
Not yet tested in other applications.
Restrictions For Research Use only
Format Liquid
Reconstitution Reconstitute in 100 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage -20 °C
Storage Comment Maintain the reconstituted antibodies frozen at -20 °C for long term storage and refrigerated at 2-8 °C for a shorter term.
Expiry Date 12 months
Supplier Images
anti-Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2) antibody IF on 1 day- old mouse cortical cell culture using Rabbit antibody to Vesicle-associated membrane protein 2 (VAMP-2, Synaptobrevin-2, VAMP2, SYB2): whole serum (ABIN351357) at 1: 2000 dilution. DAPI counter stained.
anti-Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2) antibody (2) WB on rat brain lysate using Rabbit antibody to Vesicle-associated membrane protein 2 (VAMP-2, Synaptobrevin-2, VAMP2, SYB2): whole serum (ABIN351357) at 1 : 1000 dilution. Pre-absorption of the antibody with the immunising peptide abolishes the detected band.
anti-Vesicle-Associated Membrane Protein 2 (Synaptobrevin 2) (VAMP2) antibody (3) IHC on rat brain (cortex) using Rabbit antibody to Vesicle-associated membrane protein 2 (VAMP-2, Synaptobrevin-2, VAMP2, SYB2): whole serum (ABIN351357) at 1 : 1000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).
General Archer, Ozçelik, Jahn et al.: "Structures and chromosomal localizations of two human genes encoding synaptobrevins 1 and 2." in: The Journal of biological chemistry, Vol. 265, Issue 28, pp. 17267-73, 1990 (PubMed).

Elferink, Trimble, Scheller: "Two vesicle-associated membrane protein genes are differentially expressed in the rat central nervous system." in: The Journal of biological chemistry, Vol. 264, Issue 19, pp. 11061-4, 1989 (PubMed).

Kutay, Ahnert-Hilger, Hartmann et al.: "Transport route for synaptobrevin via a novel pathway of insertion into the endoplasmic reticulum membrane." in: The EMBO journal, Vol. 14, Issue 2, pp. 217-23, 1995 (PubMed).

Jagadish, Fernandez, Hewish et al.: "Insulin-responsive tissues contain the core complex protein SNAP-25 (synaptosomal-associated protein 25) A and B isoforms in addition to syntaxin 4 and synaptobrevins 1 and 2." in: The Biochemical journal, Vol. 317 ( Pt 3), pp. 945-54, 1996 (PubMed).

Martin, Shewan, Millar et al.: "Vesicle-associated membrane protein 2 plays a specific role in the insulin-dependent trafficking of the facilitative glucose transporter GLUT4 in 3T3-L1 adipocytes." in: The Journal of biological chemistry, Vol. 273, Issue 3, pp. 1444-52, 1998 (PubMed).

Sutton, Fasshauer, Jahn et al.: "Crystal structure of a SNARE complex involved in synaptic exocytosis at 2.4 A resolution." in: Nature, Vol. 395, Issue 6700, pp. 347-53, 1998 (PubMed).

Hanson, Stevens: "Cocrystal structure of synaptobrevin-II bound to botulinum neurotoxin type B at 2.0 A resolution." in: Nature structural biology, Vol. 7, Issue 8, pp. 687-92, 2000 (PubMed).

Hosts (34), (33), (2), (1), (1), (1)
Reactivities (62), (32), (24), (12), (12), (12), (12), (12), (1), (1), (1), (1), (1), (1)
Applications (49), (30), (15), (10), (10), (10), (4), (3), (2), (2), (2), (1)
Conjugates (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (8), (7), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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