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Vesicle-Associated Membrane Protein 3 (VAMP3) antibody

Details for Product No. ABIN351363, Supplier: Log in to see
Antigen
  • zgc:73378
  • wu:fj59a03
  • ceb
  • vamp-3
  • CEB
  • D130027G05Rik
  • VAMP-3
  • zgc:86726
Reactivity
Human, Rat (Rattus), Mouse (Murine)
53
16
13
2
Host
Sheep
43
10
2
Clonality
Polyclonal
Conjugate
Un-conjugated
3
2
2
2
2
2
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
48
25
20
6
6
4
1
1
1
Supplier
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Immunogen A synthetic peptide from human VAMP3 conjugated to an immunogenic carrier protein was used as the antigen.
Specificity Specific for VAMP3.
Purification Whole serum
Alternative Name VAMP 3 (VAMP3 Antibody Abstract)
Background Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. This gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Because of its high homology to other known VAMPs, its broad tissue distribution, and its subcellular localization, the protein encoded by this gene was shown to be the human equivalent of the rodent cellubrevin. In platelets the protein resides on a compartment that is not mobilized to the plasma membrane on calcium or thrombin stimulation.
Function: Trafficking protein from a constitutively recycling pathway.
Subcellular location: Membrane, Single-pass type IV membrane protein. Cell junction, synapse, synaptosome. Also known as: VAMP-3, VAMP3, Synaptobrevin-3, Cellubrevin, SYB3, CEB.
Pathways
Application Notes A dilution of 1 : 300 to 1 : 2000 is recommended.
The optimal dilution should be determined by the end user.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.
Handling Advice Avoid freeze and thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date 12 months
Background publications Matsuoka, Ballif, Smogorzewska et al.: "ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." in: Science (New York, N.Y.), Vol. 316, Issue 5828, pp. 1160-6, 2007 (PubMed).

Gregory, Barlow, McLay et al.: "The DNA sequence and biological annotation of human chromosome 1. ..." in: Nature, Vol. 441, Issue 7091, pp. 315-21, 2006 (PubMed).

Carninci, Kasukawa, Katayama et al.: "The transcriptional landscape of the mammalian genome. ..." in: Science (New York, N.Y.), Vol. 309, Issue 5740, pp. 1559-63, 2005 (PubMed).

Gevaert, Goethals, Martens et al.: "Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides." in: Nature biotechnology, Vol. 21, Issue 5, pp. 566-9, 2003 (PubMed).

Yang, Mora, Ryder et al.: "VAMP3 null mice display normal constitutive, insulin- and exercise-regulated vesicle trafficking." in: Molecular and cellular biology, Vol. 21, Issue 5, pp. 1573-80, 2001 (PubMed).

Bernstein, Whiteheart: "Identification of a cellubrevin/vesicle associated membrane protein 3 homologue in human platelets." in: Blood, Vol. 93, Issue 2, pp. 571-9, 1999 (PubMed).

McMahon, Ushkaryov, Edelmann et al.: "Cellubrevin is a ubiquitous tetanus-toxin substrate homologous to a putative synaptic vesicle fusion protein." in: Nature, Vol. 364, Issue 6435, pp. 346-9, 1993 (PubMed).