Vesicle-Associated Membrane Protein 7 (VAMP7) antibody

Antigen: Vesicle-Associated Membrane Protein 7 (VAMP7)

Synonyms: SYBL1, VAMP-7, TI-VAMP, MGC56506, zgc:56506, sybl1, MGC83870, VAMP7

Clonality: Polyclonal

Host: Sheep

Reactivity: Please enquire

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN351366

Additional Information

Alternative name: Vesicle-associated membrane protein 7 (VAMP-7, VAMP7, SYBL1)

UniProt: P51809

Immunogen: A synthetic peptide from human Vesicle-associated membrane protein 7 (VAMP-7, VAMP7, SYBL1) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in many species including rat, mouse, chimpanzee and xenopus.

Format: Lyophilized

Description: FUNCTION: Involved in the targeting and/or fusion of transport vesicles to their target membrane during transport of proteins from the early endosome to the lysosome. Required for heterotypic fusion of late endosomes with lysosomes and homotypic lysosomal fusion. Required for calcium regulated lysosomal exocytosis. Involved in the export of chylomicrons from the endoplasmic reticulum to the cis Golgi. Required for exocytosis of mediators during eosinophil and neutrophil degranulation, and target cell killing by natural killer cells. Required for focal exocytosis of late endocytic vesicles during phagosome formation. SUBUNIT: Component of the SNARE complex composed of STX4,SNAP23 and VAMP7 that binds SYT7 during lysosomal exocytosis. Component of the SNARE complex composed of STX7, STX8, VAMP7 and VTI1B that is required for heterotypic fusion of late endosomes with lysosomes in liver cells. SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle membrane; Single-pass type IV membrane protein. Golgi apparatus, trans-Golgi network membrane; Single-pass type IV membrane protein. Late endosome membrane; Single-pass type IV membrane protein. Lysosome membrane; Single-pass type IV membrane protein. Endoplasmic reticulum membrane; Single-pass type IV membrane protein. Cytoplasmic vesicle, phagosome membrane; Single-pass type IV membrane protein. TISSUE SPECIFICITY: Detected in all tissues tested. Also known as: Vesicle-associated membrane protein 7, VAMP7, SYBL1, VAMP-7, Synaptobrevin-like protein 1, Tetanus-insensitive VAMP, Ti-VAMP.

Specificity: Appears to be specific for VAMP7.

Application Details

Application Notes: IHC, WB. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

DEsposito, Ciccodicola, Gianfrancesco et al.: "A synaptobrevin-like gene in the Xq28 pseudoautosomal region undergoes X inactivation." in: Nature genetics, Vol. 13, Issue 2, pp. 227-9, 1996 (PubMed).

Ciccodicola, DEsposito, Esposito et al.: "Differentially regulated and evolved genes in the fully sequenced Xq/Yq pseudoautosomal region." in: Human molecular genetics, Vol. 9, Issue 3, pp. 395-401, 2000 (PubMed).

Hibi, Hirashima, Nakanishi: "Rat basophilic leukemia cells express syntaxin-3 and VAMP-7 in granule membranes." in: Biochemical and biophysical research communications, Vol. 271, Issue 1, pp. 36-41, 2000 (PubMed).

Ward, Pevsner, Scullion et al.: "Syntaxin 7 and VAMP-7 are soluble N-ethylmaleimide-sensitive factor attachment protein receptors required for late endosome-lysosome and homotypic lysosome fusion in alveolar macrophages." in: Molecular biology of the cell, Vol. 11, Issue 7, pp. 2327-33, 2000 (PubMed).

Martinez-Arca, Rudge, Vacca et al.: "A dual mechanism controlling the localization and function of exocytic v-SNAREs." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 15, pp. 9011-6, 2003 (PubMed).

Braun, Fraisier, Raposo et al.: "TI-VAMP/VAMP7 is required for optimal phagocytosis of opsonised particles in macrophages." in: The EMBO journal, Vol. 23, Issue 21, pp. 4166-76, 2004 (PubMed).

Siddiqi, Mahan, Siddiqi et al.: "Vesicle-associated membrane protein 7 is expressed in intestinal ER." in: Journal of cell science, Vol. 119, Issue Pt 5, pp. 943-50, 2006 (PubMed).