Vesicular Acetylcholine Transporter (VACHT) antibody

Antigen: Vesicular Acetylcholine Transporter (VACHT)

Synonyms: vAChTP, Ci-ChAT, Slc18a3, Ci-vAChTP, MGC153442, SLC18A3, VACHT, LOC100226071, VAChT, rVAT, VACht, MGC64220, MGC12716, VAT, CG12345, CG32848, CT41182, DmelCG32848, vacht, vAChT, Vacht

Clonality: Polyclonal

Host: Goat

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN351373

Additional Information

Swiss-Prot: Q16572

Immunogen: A synthetic peptide from human VACHT conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in rat and mouse.

Format: Lyophilized

Description: FUNCTION: Involved in acetylcholine transport into synaptic vesicles. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: High expression in all major cholinergic cell groups, including peripheral postganglionic parasympathetic cells, preganglionic sympathetic and parasympathetic cells, ventral spinal cord and brainstem motoneurons, cell groups in the basal forebrain, the habenula and the corpus striatum. Weakly expressed in the cortex and hippocampus. VACHT is also present in theolfactory bulb, nucleus of the lateralolfactory tract,olfactory tubercle. SIMILARITY: Belongs to the major facilitator superfamily. Vesicular transporter family. Also known as: Vesicular acetylcholine transporter, solute carrier family 18 member 3, SLC18A3, MGC12716.

Specificity: Appears to be specific for VACHT.

Application Details

Application Notes: IHC, WB. A dilution of 1: 300 to 1: 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Roghani, Feldman, Kohan et al.: "Molecular cloning of a putative vesicular transporter for acetylcholine." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 22, pp. 10620-4, 1994 (PubMed).

Erickson, Varoqui, Schaefer et al.: "Functional identification of a vesicular acetylcholine transporter and its expression from a "cholinergic" gene locus." in: The Journal of biological chemistry, Vol. 269, Issue 35, pp. 21929-32, 1994 (PubMed).

Bejanin, Cervini, Mallet et al.: "A unique gene organization for two cholinergic markers, choline acetyltransferase and a putative vesicular transporter of acetylcholine." in: The Journal of biological chemistry, Vol. 269, Issue 35, pp. 21944-7, 1994 (PubMed).

Ichikawa, Ajiki, Matsuura et al.: "Localization of two cholinergic markers, choline acetyltransferase and vesicular acetylcholine transporter in the central nervous system of the rat: in situ hybridization histochemistry and immunohistochemistry." in: Journal of chemical neuroanatomy, Vol. 13, Issue 1, pp. 23-39, 1997 (PubMed).