VGluT1 (VGLU1, Slc17a7, Bnpi, Vesicular glutamate transporter 1) (Cytoplasmic Loop) antibody

Antigen: VGluT1 (VGLU1, Slc17a7, Bnpi, Vesicular glutamate transporter 1)

Binding Site: Cytoplasmic Loop

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN351379

Additional Information

UniProt: Q62634

Immunogen: A synthetic peptide from a cytoplasmic domain of rat VGluT1 (VGLU1, Slc17a7, Bnpi, Vesicular glutamate transporter 1) conjugated to an immunogenic carrier protein was used as the immunogen. The antigen shares 92% identity with mouse's and 90% with human's.

Format: Lyophilized

Isotype: IgG

Description: FUNCTION: Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate. SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Membrane; Multi-pass membrane protein. Cell junction, synapse, synaptosome. Also known as: Vesicular glutamate transporter 1, VGlU1, Solute carrier family 17 member 7, Brain-specific Na(+)-dependent inorganic phosphate cotransporter, Bnpi.

Specificity: Appears to be specific for VGLUT1 (both isoforms 1 and 2).

Application Details

Application Notes: IHC, WB, Flow Cyt. A concentration of 10-50 µg/ml is recommended. The optimal concentration should be determined by the end user. Not yet tested in other applications.

Purity: IgG

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Ni, Rosteck, Nadi et al.: "Cloning and expression of a cDNA encoding a brain-specific Na(+)-dependent inorganic phosphate cotransporter." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 12, pp. 5607-11, 1994 (PubMed).

Takamori, Rhee, Rosenmund et al.: "Identification of a vesicular glutamate transporter that defines a glutamatergic phenotype in neurons." in: Nature, Vol. 407, Issue 6801, pp. 189-94, 2000 (PubMed).

Herzog, Bellenchi, Gras et al.: "The existence of a second vesicular glutamate transporter specifies subpopulations of glutamatergic neurons." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 21, Issue 22, pp. RC181, 2001 (PubMed).

Takamori, Rhee, Rosenmund et al.: "Identification of differentiation-associated brain-specific phosphate transporter as a second vesicular glutamate transporter (VGLUT2)." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 21, Issue 22, pp. RC182, 2001 (PubMed).

Nogami, Ogasawara, Mimura et al.: "Developmentally-regulated expression of tissue-specific splice variant of rat vesicular glutamate transporter 1 in retina and pineal gland." in: Journal of neurochemistry, Vol. 99, Issue 1, pp. 142-53, 2006 (PubMed).