Solute Carrier Family 17 (Sodium-Dependent Inorganic Phosphate Cotransporter), Member 6 (SLC17A6) (N-Term) antibody

Antigen: Solute Carrier Family 17 (Sodium-Dependent Inorganic Phosphate Cotransporter), Member 6 (SLC17A6)

Synonyms: DNPI, VGLUT2, Dnpi, Vglut2, 2900073D12Rik, vglut2, slc17a6, MGC83509, SLC17A6, VGluT2, MGC166269

Epitope: N-Term

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN351382

Additional Information

Alternative name: VGluT2 (VGLU2, Vesicular glutamate transporter 2) (n-terminal region)

Swiss-Prot: Q9JI12

Immunogen: A synthetic peptide from the n-terminal part of rat, mouse VGluT2 (VGLU2, Slc17a6, Dnpi, Vesicular glutamate transporter 2) conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: FUNCTION: Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate. SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Membrane; Multi-pass membrane protein. Cell junction, synapse, synaptosome. TISSUE SPECIFICITY: Expressed in brain. Expressed in hippocampal neurons (at protein level). DEVELOPMENTAL STAGE: Expressed in brain throughout development. Transiently expressed in hippocampal neurons during the first week after birth, with expression decreasing thereafter. MISCELLANEOUS: Mice lacking this protein exhibit an elevated rate of perinatal lethality. Surviving animals display a strong reduction in evoked glutamergic responses in thalamic neurons. Reduction of protein level in homozygous and heterozygous knockouts leads to a graded reduction in the amplitude of the postsynaptic response to single vesicle fusion in thalamic neurons, consistent with a role for this protein in determining quantal size. Also known as: Vesicular glutamate transporter 2, VGlU2, Solute carrier family 17 member 6, Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter, Differentiation-associated BNPI, Slc17a6, Dnpi.

Specificity: Appears to be specific for VGLUT2.

Application Details

Application Notes: IHC, WB. A dilution of 1: 300 to 1: 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Bai, Xu, Collins et al.: "Molecular and functional analysis of a novel neuronal vesicular glutamate transporter." in: The Journal of biological chemistry, Vol. 276, Issue 39, pp. 36764-9, 2001 (PubMed).

Schaefer, Varoqui, Defamie et al.: "Molecular cloning and functional identification of mouse vesicular glutamate transporter 3 and its expression in subsets of novel excitatory neurons." in: The Journal of biological chemistry, Vol. 277, Issue 52, pp. 50734-48, 2002 (PubMed).

Wojcik, Rhee, Herzog et al.: "An essential role for vesicular glutamate transporter 1 (VGLUT1) in postnatal development and control of quantal size." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 18, pp. 7158-63, 2004 (PubMed).

Fremeau, Kam, Qureshi et al.: "Vesicular glutamate transporters 1 and 2 target to functionally distinct synaptic release sites." in: Science (New York, N.Y.), Vol. 304, Issue 5678, pp. 1815-9, 2004 (PubMed).

Carninci, Kasukawa, Katayama et al.: "The transcriptional landscape of the mammalian genome." in: Science (New York, N.Y.), Vol. 309, Issue 5740, pp. 1559-63, 2005 (PubMed).

Herzog, Takamori, Jahn et al.: "Synaptic and vesicular co-localization of the glutamate transporters VGLUT1 and VGLUT2 in the mouse hippocampus." in: Journal of neurochemistry, Vol. 99, Issue 3, pp. 1011-8, 2006 (PubMed).

Moechars, Weston, Leo et al.: "Vesicular glutamate transporter VGLUT2 expression levels control quantal size and neuropathic pain." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 26, Issue 46, pp. 12055-66, 2006 (PubMed).