Solute Carrier Family 17 (Sodium-Dependent Inorganic Phosphate Cotransporter), Member 8 (SLC17A8) (C-Term) antibody

Antigen: Solute Carrier Family 17 (Sodium-Dependent Inorganic Phosphate Cotransporter), Member 8 (SLC17A8)

Synonyms: Vglut3, BC042593, DFNA25, VGLUT3, vglut3, si:ch211-264e16.4

Epitope: C-Term

Clonality: Polyclonal

Host: Rabbit

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN351384

Additional Information

Alternative name: VGluT3 (Vesicular glutamate transporter 3) (c-terminal region)

Swiss-Prot: Q8BFU8

Immunogen: A synthetic peptide from the c-terminal region of mouse VGLUT3 conjugated to an immunogenic carrier protein was used as the immunogen.

Format: Lyophilized

Description: FUNCTION: Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate. SUBCELLULAR LOCATION: Cytoplasmic vesicle › secretory vesicle › synaptic vesicle membrane. Membrane; Multi-pass membrane proteinPotential. Cell junction › synapse › synaptosome. TISSUE SPECIFICITY: Expressed in brain, kidney and liver. Expressed within the amygdala, brainstem, cerberal cortex, dorsal root ganglia, dorsal spinal cord, hippocampus, hypothalamus, retina, striatum and ventral spinal cord. Expressed within neurons of the caudate-putamen,olfactory tubercle, nucleus accumbens, hippocampus, interpeduncular nucleus and dorsal and medial raphe nuclei. Expressed in inner hair cells of the ear. Expressed at synaptic terminals within the lateral superior olive (LSO), a nucleus of the mammalian sound localization system, and in the medial nucleus of the trapezoid body (MNTB), which provides inhibitory input to the LSO. Also known as: Vesicular glutamate transporter 3, Solute carrier family 17 member 8.

Specificity: Appears to be specific for VGLUT3.

Application Details

Application Notes: IHC, WB. A dilution of 1: 300 to 1: 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Purity: whole serum

Storage: Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Restrictions: For Research Use only

Images

WB on mouse brain lysate (10% gel, 1:29. Reduced, denatured) using Rabbit antibody to c-terminal region of mouse VGluT3 (Vesicular glutamate transporter 3): whole serum (ABIN351384) at 1: 500 dilution. Blocked with 1% LFDM for 15 minutes at room temperature with shake, primary antibody incubated for 15 minutes at room temperature, washed 3 times with PBS (0.1 x), 5 minutes each. Secondary antibody was also incubated for 15 minutes at room temperature.

Publications

Publications: Schaefer, Varoqui, Defamie et al.: "Molecular cloning and functional identification of mouse vesicular glutamate transporter 3 and its expression in subsets of novel excitatory neurons." in: The Journal of biological chemistry, Vol. 277, Issue 52, pp. 50734-48, 2002 (PubMed).

Mackenzie, Illing, Morris et al.: "Analysis of a vesicular glutamate transporter (VGLUT2) supports a cell-leakage mode in addition to vesicular packaging." in: Neurochemical research, Vol. 33, Issue 2, pp. 238-47, 2008 (PubMed).

Seal, Akil, Yi et al.: "Sensorineural deafness and seizures in mice lacking vesicular glutamate transporter 3." in: Neuron, Vol. 57, Issue 2, pp. 263-75, 2008 (PubMed).

Gras, Amilhon, Lepicard et al.: "The vesicular glutamate transporter VGLUT3 synergizes striatal acetylcholine tone." in: Nature neuroscience, Vol. 11, Issue 3, pp. 292-300, 2008 (PubMed).

Ruel, Emery, Nouvian et al.: "Impairment of SLC17A8 encoding vesicular glutamate transporter-3, VGLUT3, underlies nonsyndromic deafness DFNA25 and inner hair cell dysfunction in null mice." in: American journal of human genetics, Vol. 83, Issue 2, pp. 278-92, 2008 (PubMed).